Current Microbiology

, Volume 22, Issue 1, pp 59–63 | Cite as

Cysteine proteinase changes during microcyst formation and germination inPolysphondylium pallidum

  • Michael J. North
  • David A. Cotter
  • Karl J. Franek


Microcyst formation inPolysphondylium pallidum WS320 was accompanied by a decrease in intracellular cysteine proteinase activity measured with the peptide nitroanilides Z-Arg-Arg-Nan and Bz-Pro-Phe-Arg-Nan. Some activity was released into the buffer, and secretion of that towards Z-Arg-Arg-Nan continued until encystment occurred. Cells grown in association withEscherichia coli had an electrophoretic proteinase pattern different from cells grown axenically. Microcysts formed from the two cell populations also had distinct proteinase patterns; those from bacterially grown cells retained significant quantities of proteinase ppCP22, whereas those derived from axenic cells were devoid of detectable proteinases. No significant changes in cysteine proteinase activities were observed during microcyst germination, although some changes in activity occurred subsequent to emergence. The results indicate that there is not a close correlation between particular cysteine proteinases and specific stages of microcyst formation. Intracellular proteinase loss and concomitant secretion are, however, processes typical of cellular slime molds developing in response to starvation.


Peptide Mold Cysteine Cysteine Proteinase Close Correlation 
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Literature Cited

  1. 1.
    Bradford M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedGoogle Scholar
  2. 2.
    Cardelli JA, Dimond RL (1988) Transport and targeting of lysosomal enzymes inDictyostelium discoideum In: Das R, Robbins P (eds), Protein transfer and organelle biogenesis. New York: Academic Press, pp 363–399Google Scholar
  3. 3.
    Cotter DA, Raper KB (1968) Factors affecting the rate of heat-induced spore germination inDictyostelium discoideum. J Bacteriol 96:86–92PubMedGoogle Scholar
  4. 4.
    Francis DW (1982)Polysphondylium and dependent sequences, In: The development ofDictyostelium discoideum. New York: Academic Press, pp 387–409Google Scholar
  5. 5.
    Free SJ, Loomis WF (1974) Isolation of mutations inDictyostelium discoideum affecting α-mannosidase. Biochimie 56:1525–1528PubMedGoogle Scholar
  6. 6.
    Kost RC, North MJ, Whyte A (1981) Acid proteinases in various species of cellular slime mold. Exp Mycol 5:269–277Google Scholar
  7. 7.
    North MJ (1985) Cysteine proteinases of cellular slime moulds. Biochem Soc Transact 13:288–290Google Scholar
  8. 8.
    North MJ, Cotter DA (1984) Proteolytic enzyme activity duringDictyostelium discoideum spore germination. Exp Mycol 8:47–54Google Scholar
  9. 9.
    North MJ, Roper AM, Walker M (1984) Increase in proteinase activity in the cellular slime mouldPolysphondylium pallidum induced by bacteria. FEMS Microbiol Lett 21:175–179Google Scholar
  10. 10.
    North MJ, Scott KI, Lockwood BC (1988) Multiple cysteine proteinase forms during the life cycle ofDictyostelium discoideum revealed by electrophoretic analysis. Biochem J 254:261–268PubMedGoogle Scholar
  11. 11.
    North MJ, Cotter DA, Franek KJ (1990)Dictyostelium discoideum spore germination: increases in proteinase activity are not directly coupled to the emergence of myxamoebae. J Gen Microbiol 136:835–840Google Scholar
  12. 12.
    North MJ, Franek KJ, Cotter DA (1990) Differential secretion ofDictyostelium discoideum proteinases. J Gen Microbiol 136:827–833Google Scholar
  13. 13.
    North MJ, Cotter DA, Franek KJ (1990) Cysteine proteinase changes during macrocyst formation inDictyostelium mucoroides. SubmittedGoogle Scholar
  14. 14.
    O'Day DH (1976) Acid protease activity during germination of microcysts of the cellular slime moldPolysphondylium pallidum. J Bacteriol 125:8–13PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Michael J. North
    • 1
  • David A. Cotter
    • 2
  • Karl J. Franek
    • 2
  1. 1.Department of Biological and Molecular Sciences, School of Natural SciencesUniversity of StirlingStirlingScotland
  2. 2.Department of Biological SciencesUniversity of WindsorWindsorCanada

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