Current Microbiology

, Volume 22, Issue 1, pp 15–19 | Cite as

Escherichia coli-Anacystis nidulans plasmid shuttle vecotrs containing the PL promoter from bacteriophage lambda

  • Margaret Y. Gruber
  • Bernard R. Glick
  • John E. Thompson


Escherichia coli-Anacystis nidulans shuttle vectors pHIEX14, pSMG1, and pANHI, containing the leftward promoter, PL, of bacteriophage lambda and the gene for the temperaturesensitive repressor, cI857, were constructed and used to transformA. nidulans. The transformation efficiencies and restriction endonuclease maps of these plasmids are reported. The use of these shuttle vectors should allow temperature regulation of foreign gene expression inA. nidulans.


Gene Expression Restriction Endonuclease Temperature Regulation Transformation Efficiency Foreign Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Allen MM (1968) Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4:1–4Google Scholar
  2. 2.
    Bernard H-U, Remaut E, Herschfield MV, Das HK, Helinski DR, Yanofsky C, Franklin N (1979) Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda PL promoter. Gene 5:59–76PubMedGoogle Scholar
  3. 3.
    Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523PubMedGoogle Scholar
  4. 4.
    Bittner M, Kufkerer P, Morris CF (1980) Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. Anal Biochem 102:459–471PubMedGoogle Scholar
  5. 5.
    Buzby JS, Porter RD, Stevens SE Jr (1985) Expression of theEscherichia coli lacZ gene on a plasmid vector in a cyanobacterium. Science 230:805–807PubMedGoogle Scholar
  6. 6.
    Daniell H, Sarojini G, McFadden BA (1986) Transformation of the cyanobacteriumAnacystis nidulans 6301 with theEscherichia coli plasmid pBR322. Proc Natl Acad Sci USA 83:2546–2550PubMedGoogle Scholar
  7. 7.
    Denhard DT (1986) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646Google Scholar
  8. 8.
    Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  9. 9.
    Friedberg D, Seijffers J (1983) A new hybrid plasmid capable of transformingEscherichia coli andAnacystic nidulans. Gene 22:267–275PubMedGoogle Scholar
  10. 10.
    Friedberg D, Seijffers J (1986) Controlled gene expression utilizing Lambda phage regulatory signals in a cyanobacterium host. Mol Gen Genet 203:505–510PubMedGoogle Scholar
  11. 11.
    Gallagher ML, Burke WF Jr (1987) Construction of hybrid plasmid vectors for cloning inEscherichi coli, Bacillus subtilis and the cyanobacteriumAnacystis nidulans. Curr Microbiol 16:43–47Google Scholar
  12. 12.
    Gendel S, Straus N, Pulleyblank D, Williams J (1983) Shuttle cloning vectors for the cyanobacteriumAnacystis nidulans. J Bacteriol 156:148–154PubMedGoogle Scholar
  13. 13.
    Golden SS, Sherman LA (1983) A hybrid plasmid is a stable cloning vector for the cyanobacteriumAnacystis nidulans R2. J Bacteriol 155:966–972PubMedGoogle Scholar
  14. 14.
    Golden SS, Sherman LA (1984) Optimal conditions for genetic transformation of the cyanobacteriumAnacystis nidulans R2. J Bacteriol 158:36–42PubMedGoogle Scholar
  15. 15.
    Gruber MY, Glick BR, Thompson JE, Gendel SM (1987)In vitro expression of a cyanobacterial plasmid. Curr Microbiol 15:265–268Google Scholar
  16. 16.
    Gruber MY, Glick BR, Thompson JE (1990) Cloned Mn-superoxide dismutase reduces oxidative stress inEscherichia coli andAnacystis nidulans. Proc Natl Acad Sci USA 87:2608–2612PubMedGoogle Scholar
  17. 17.
    Kuhlemeier CJ, Borrias WE, van den Hondel CAMJJ, van Arkel GA (1981) Vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation toEscherichia coli K12 andAnacystis nidulans R2. Mol Gen Genet 184:249–254PubMedGoogle Scholar
  18. 18.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedGoogle Scholar
  19. 19.
    Lau RH, Straus NA (1985) Versatile shuttle cloning vectors for the unicellular cyanobacteriumAnacystis nidulans R2. FEMS Microbiol Lett 27:253–256Google Scholar
  20. 20.
    Leemans R, Remaut E, Fiers W (1987) A broad-host-range expression vector based on the PL promoter of coliphage lambda: regulated synthesis of human interleukin 2 inErwinia andSerratia species. J Bacteriol 169:1899–1904PubMedGoogle Scholar
  21. 21.
    de Lorimier R, Guglielmi G, Bryant DA, Stevens SE Jr (1987) Functional expression of plastid allophycocyanin genes in a cyanobacterium. J Bacteriol 169:1830–1835PubMedGoogle Scholar
  22. 22.
    Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.Google Scholar
  23. 23.
    Miller JH (ed) (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.Google Scholar
  24. 24.
    See YP, Glick BR (1982) Analysis of the expression of cloned genes using anEscherichia coli cell-free system. Can J Biochem 60:1095–1100PubMedGoogle Scholar
  25. 25.
    Tandeau de Marsac N, de la Torre F, Szulmajster J (1987) Expression of the larvicidal gene ofBacillus sphaericus 1593M in the cyanobacteriumAnacystic nidulans R2. Mol Gen Genet 209:396–398PubMedGoogle Scholar
  26. 26.
    Williams JGK, Szalay AA (1983) Stable integration of foreign DNA into the chromosome of the cyanobacteriumSynechococcus R2. Gene 24:37–51PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Margaret Y. Gruber
    • 2
  • Bernard R. Glick
    • 1
  • John E. Thompson
    • 1
  1. 1.107 Science CrescentAgriculture Canada Research StationSaskatoonCanada
  2. 2.Department of BiologyUniversity of WaterlooWaterlooCanada

Personalised recommendations