Current Microbiology

, Volume 22, Issue 1, pp 15–19 | Cite as

Escherichia coli-Anacystis nidulans plasmid shuttle vecotrs containing the PL promoter from bacteriophage lambda

  • Margaret Y. Gruber
  • Bernard R. Glick
  • John E. Thompson
Article

Abstract

Escherichia coli-Anacystis nidulans shuttle vectors pHIEX14, pSMG1, and pANHI, containing the leftward promoter, PL, of bacteriophage lambda and the gene for the temperaturesensitive repressor, cI857, were constructed and used to transformA. nidulans. The transformation efficiencies and restriction endonuclease maps of these plasmids are reported. The use of these shuttle vectors should allow temperature regulation of foreign gene expression inA. nidulans.

Keywords

Gene Expression Restriction Endonuclease Temperature Regulation Transformation Efficiency Foreign Gene 

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Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Margaret Y. Gruber
    • 2
  • Bernard R. Glick
    • 1
  • John E. Thompson
    • 1
  1. 1.107 Science CrescentAgriculture Canada Research StationSaskatoonCanada
  2. 2.Department of BiologyUniversity of WaterlooWaterlooCanada

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