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Spontaneous temperature-sensitive mutants ofPseudomonas cepacia: Isolation and utilization

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Abstract

Pseudomonas cepacia strain 224c was subjected to treatment with two alternating cycles of penicillin andd-cycloserine in order to isolate spontaneous temperature-sensitive (ts) mutants. Of 150 surviving clones tested, ten were ts mutants; these were partially characterized, and one, Pcp-5, was chosen for experiments to determine the in vivo growth rate of the wild-type (wt) and clearance from the peritoneal cavities of mice. This mutant ceases growth almost immediately after transfer from 29°C to the nonpermissive (36°C) temperature and has a reversion rate of 10−8. In vivo experiments were performed by inoculating mixtures of Pcp-5 and wt into the peritoneal cavities of ICR mice. The ts mutant was cleared quantitatively (90% by 4 h), and the mean generation time (MGT) of the wt in the murine peritoneal cavity was 94 min.

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Authors and Affiliations

  1. Department of Microbiology, Miami University, 45056, Oxford, Ohio, USA

    Anne Morris Hooke, Renee C. Palecek, Theresa M. Palecek & Deidre R. MIller

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  1. Anne Morris Hooke
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  2. Renee C. Palecek
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  3. Theresa M. Palecek
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  4. Deidre R. MIller
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Hooke, A.M., Palecek, R.C., Palecek, T.M. et al. Spontaneous temperature-sensitive mutants ofPseudomonas cepacia: Isolation and utilization. Current Microbiology 22, 129–132 (1991). https://doi.org/10.1007/BF02105388

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  • DOI: https://doi.org/10.1007/BF02105388

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