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Cloning of promoter-active DNA sequences from the cyanobacteriumSynechocystis sp. PCC 6803 in anEscherichia coli host

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Abstract

DNA fragments fromSynechocystis sp. PCC 6803, which act as promoters inE. coli, were cloned by transcriptional gene fusion togalK. They were characterized by determining their relative strength. Shot-gun cloning ofSynechocystis DNA fragments resulted in 3% ofE. coli transformants possessing cyanobacterial DNA sequences with promoter activity. The determination of relative promoter strengths of 36 randomly selected clones with the galactokinase assay demonstrated the dominance of weak and intermediate promoters.

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Authors and Affiliations

  1. Division of Biology, University of Rostock, Germany

    Carsten Milkowski

  2. Division of Genetics, Martin Luther University, Halle, Germany

    Ariel Quiñones

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  1. Carsten Milkowski
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  2. Ariel Quiñones
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Milkowski, C., Quiñones, A. Cloning of promoter-active DNA sequences from the cyanobacteriumSynechocystis sp. PCC 6803 in anEscherichia coli host. Current Microbiology 23, 333–336 (1991). https://doi.org/10.1007/BF02104135

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  • DOI: https://doi.org/10.1007/BF02104135

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