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Catabolite repression of the colonization factor antigen I (CFA/I) operon ofEscherichia coli

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Abstract

Experiments were performed to study whether the synthesis of the fimbrial colonization factor antigen I (CFA/I) of enterotoxigenicEscherichia coli is affected by glucose. The CFA/I-producing strain H-10407 (O78:H11:CFA/I) was grown in CFA medium containing various concentrations of glucose. Addition of 1% glucose into the medium resulted in a pronounced decrease in CFA/I production by H-10407 as assessed by ELISA, hemagglutination, and electron microscopy. The repressive effect of glucose was reversed by the addition of 10 mM cAMP to the medium. Examination of the promoter sequence of thecfaA gene of the CFA/I operon revealed a consensus binding site for the catabolite activator protein-cAMP complex. With a reporter plasmid containing a fusion of thecfaA promoter, a portion of thecfaA gene, and thelacZ gene, it was shown that the activity of this promoter was influenced by glucose. In a wild-typeE. coli strain, addition of 0.5% glucose to the growth medium diminished the promoter activity more than 70%. ThecfaA promoter also exhibited a lower level of activity incya (adenyl cyclase) andcrp (cAMP receptor protein) mutants than in the wild-type strain. The addition of 10 mM cAMP resulted in a marked increase in the expression from thecfaA promoter in thecya but not in thecrp mutant. These results suggest that the suppressive effect of glucose in the CFA/I system is mediated via the mechanism of catabolite repression through thecfaA promoter of the CFA/I operon.

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Authors and Affiliations

  1. Department of Medicine, Baylor College of Medicine and Veterans Administration Medical Center, Houston, Texas, USA

    Dolores G. Evans, Doyle J. Evans Jr. & David Y. Graham

  2. Department of Pathology, Indiana University School of Medicine, 1120 South Drive, FH 419, 46202, Indianapolis, IN, USA

    Tuomo K. Karjalainen &  Chao-Hung Lee

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  1. Tuomo K. Karjalainen
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  2. Dolores G. Evans
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  3. Doyle J. Evans Jr.
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  4. David Y. Graham
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  5. Chao-Hung Lee
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Karjalainen, T.K., Evans, D.G., Evans, D.J. et al. Catabolite repression of the colonization factor antigen I (CFA/I) operon ofEscherichia coli . Current Microbiology 23, 307–313 (1991). https://doi.org/10.1007/BF02104131

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