Summary
We have prepared molecules in which a guanosine 5′-phosphate (pG) residue is attached to the 3′ terminus of a decadeoxycytidylate (pdC)10 template via diamine linkers H2N(CH2) n NH2,n=4–7. The pG residue acts as a primer and is extended very efficiently by incubation with activated pG derivatives to give products containing 6–9 G residues in >80% yield. The detailed nature of the product distribution is discussed.
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Abbreviations
- CDI:
-
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
- CIP:
-
calf intestinal alkaline phosphatase
- CPG:
-
controled-pore glass
- ImpG:
-
guanosine 5′-phosphorimidazolide
- 2-MeImpG:
-
(guanosine 5′-phosphor)-2-methylimidazolide
- 2-MeImpA:
-
(adenosine 5′-phosphor)-2-methylimidazolide
References
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S n , d(C10)3′p-NH-(CH2) n -NH-5′pG; A n , d(C10)3′p-NH-(CH2) n -NH2
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Rodriguez, L., Orgel, L.E. Template-directed extension of a guanosine 5′-phosphate covalently attached to an oligodeoxycytidylate template. J Mol Evol 33, 477–482 (1991). https://doi.org/10.1007/BF02102800
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DOI: https://doi.org/10.1007/BF02102800