Abstract
The effects of chilling on ethylene production by leaf discs and whole plants of bean (chilling-sensitive) and pea (chilling-tolerant) were studied. When pea or bean leaf discs were excised and incubated at 25°C, transient increases in ethylene production and 1-aminocyclopropane-1-carboxylic acid (ACC) accumulation were observed. Both pea and bean discs kept at 5°C evolved little ethylene, but levels of ACC increased in pea discs and not in bean discs. When discs of either species were chilled at 5°C immediately after excision and then transferred to 25°C 9 h later, increases in their ACC levels and ethylene production rates were observed. Discs were also incubated at 25°C for 12 h to allow excision-induced ethylene production to subside and then chilled at 5°C. Nine hours later, these discs were transferred to 25°C, and an increase in ethylene production was observed. These data indicate that chilling suppresses excision-induced ethylene production and enhances the production of ethylene after transfer to 25°C. Chilling of whole plants resulted in increased production of ethylene and ACC in the chilling-sensitive bean but not in the chilling-tolerant pea. Treatment of bean plants with the ethylene antagonists silver thiosulfate, norbornadiene, or aminooxyacetic acid, or of pea plants with ethylene, did not affect the appearance of chilling injury symptoms, indicating that ethylene does not induce injury symptoms and may not have an adaptive role in chilling stress.
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Beyer EM (1979) Effect of silver ion, carbon dioxide, and oxygen on ethylene action and metabolism. Plant Physiol 63:169–173
Chan HT, Sanxter S, Couey HM (1985) Electrolyte leakage and ethylene production induced by chilling injury of papayas. HortScience 20:1070–1072
Chen Y, Patterson BD (1985) Ethylene and 1-aminocyclopropane-1-carboxylic acid as indicators of chilling sensitivity in various plant species. Aust J Plant Physiol 12:377–385
Cooper WC, Rasmussen GK, Waldon ES (1969) Ethylene stimulated by chilling inCitrus andPersea sp. Plant Physiol 44:1194–1196
Field RJ (1981) The effect of low temperature on ethylene production by leaf tissue ofPhaseolus vulgaris L. Ann Bot 47:215–223
Field RJ (1984) The role of 1-aminocyclopropane-1-carboxylic acid in the control of low temperature induced ethylene production in leaf tissue ofPhaseolus vulgaris L. Ann Bot 54:61–67
Lizada C, Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem 100:140–145
Sisler EC, Yang SF (1984) Anti-ethylene effects ofcis-2-butene and cyclic olefins. Phytochemistry 23:2765–2768
Veen H (1983) Silver thiosulfate: An experimental tool in plant science. Sci Hortic 20:211–224
Wang CY, Adams DO (1980) Ethylene production by chilled cucumbers (Cucumis sativus L.). Plant Physiol 66:841–843
Wang CY, Adams DO (1982) Chilling-induced ethylene production in cucumbers (Cucumis sativus L.). Plant Physiol 69:424–427
Yu YB, Adams DO, Yang SF (1979) 1-Aminocyclopropane-carboxylate synthase, a key enzyme in ethylene biosynthesis. Arch Biochem Biophys 198:280–286
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Tong, C.B.S., Yang, S.F. Chilling-induced ethylene production by beans and peas. J Plant Growth Regul 6, 201–208 (1987). https://doi.org/10.1007/BF02102548
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DOI: https://doi.org/10.1007/BF02102548