Abstract
In vitro flower bud initiation and development depend on the presence of two hormones in the culture medium—auxin (NAA) and cytokinin (BAP). The uptake of both NAA and BAP by the explants was shown to be proportional to the concentrations supplied in the medium over a period of 4 days after the onset of culture. However, when supplied at equal concentrations for 24 h, the NAA uptake was up to 10-fold higher than the BAP uptake. Both hormones are rapidly metabolized by the explants. Nevertheless, the concentrations of free hormones inside the explants appeared to be high and in the case of NAA exceeded the concentration in the medium by more than 1 order of magnitude within 24 h. Apparently flower bud initiation in tobacco explants requires relatively high concentrations free NAA and BAP in the tissue maintained by a continuous supply in the medium. There are at present no indications that the products of hormone metabolism are directly involved in bud formation.
References
Barendse GWM, Croes AF, Bosveld M (1986) Uptake and metabolism of NAA and BAP with respect toin vitro flower bud formation ofNicotiana tabacum. Proceedings of the 13th Annual Meeting, Plant Growth Regulation Society of America, St. Petersburg Beach, FL 1986:202–207
Caboche M, Aranda G, Poll AM, Huet J-C, Legnay J-J (1984) Auxin conjugation by tobacco mesophyll protoplasts. Plant Physiol 75:54–59
Cohen JD, Bandurski RS (1982) Chemistry and physiology of the bound auxins. Annu Rev Plant Physiol 33:403–430
Goren R, Bukovac MJ (1973) Mechanism of naphthaleneacetic acid conjugation. Plant Physiol 51:907–913
Kazemie M, Klämbt D (1969) Untersuchungen zur Wirkungsweise der Aminosäurekonjugate der Auxine in wachsenden Geweben. Physiol Plant 22:489–496
Letham DS, Palni LMS (1983) The biosynthesis and metabolism of cytokinins. Annu Rev Plant Physiol 34:163–197
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Rubery PH (1978) Hydrogen ion dependence of carrier-mediated auxin uptake by suspension-cultured crown gall cells. Planta 142:203–206
Rubery PH, Sheldrake AR (1973) Effect of pH and surface charge on cell uptake of auxin. Nature New Biol 244:285–288
Rubery PH, Sheldrake AR (1974) Carrier-mediated auxin transport. Planta 118:101–121
Van den Ende G, Barendse GWM, Kemp A, Croes AF (1984a) The role of glucose on flower bud formation in thin-layer tissue cultures ofNicotiana tabacum L. J Exp Bot 35:1853–1859
Van den Ende G, Croes AF, Kemp A, Barendse GWM (1984b) Development of flower buds in thin-layer cultures of floral stalk tissue from tobacco: Role of hormones in different stages. Physiol Plant 61:114–118
Van den Ende G, Croes AF, Kemp A, Barendse GWM, Kroh M (1984c) Floral morphogenesis in thin-layer tissue cultures ofNicotiana tabacum. Physiol Plant 62:83–88
Vijayaraghavan SJ, Pengelly WL (1986) Bound auxin metabolism in cultured crown-gall tissues of tobacco. Plant Physiol 80:315–321
Zenk MH (1962) Aufnahme und Stoffwechsel von α-naphthyl-Essigsaüre durch Erbsenepicotyle. Planta 58:75–94
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Barendse, G.W.M., Croes, A.F., Bosveld, M. et al. Uptake and metabolism of NAA and BAP in explants of tobacco in relation toin vitro flower bud formation. J Plant Growth Regul 6, 193–200 (1987). https://doi.org/10.1007/BF02102547
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DOI: https://doi.org/10.1007/BF02102547