Comparative analysis of alternating purine-pyrimidine tracts and potential Z-DNA sequences in DNA plant viruses
- 35 Downloads
The DNAs of several plant viruses were analyzed for the presence of alternating purine-pyrimidine sequences that can potentially undergo B to Z transition. The DNA of the caulimoviruses (plant retroviruses) was compared with that of the geminiviruses, with the cDNA of an RNA plant virus, and with several computer-generated random sequences. Our analysis indicates that potential Z-DNA sites tend to be restricted in the DNA of the caulimoviruses, whereas the same does not occur significantly in the other viral DNAs examined. This result is discussed in relation to the mode of replication of the caulimoviral DNA and offers additional evidence of the existence of selection processes regulating the frequency and distribution of the Z-DNA sites in the different genomes.
Key wordsDNA plant virus Z-DNA Supercoiled DNA Caulimovirus Geminivirus Viral evolution Reverse transcription
Unable to display preview. Download preview PDF.
- Bouzoubaa S, Ziegler V, Beck D, Guilley H, Richards K, Jonard G (1986) Nucleotide sequence of beet necrotic yellow vein virus RNA-2. J Gen Virol 67:1689–1700Google Scholar
- Hamilton WDO, Stein VE, Coutts RHA, Buck KW (1984) Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J 9:2197–2205Google Scholar
- Howarth AJ, Caton J, Bossert M, Goodman RM (1985) Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proc Natl Acad Sci USA 82:3572–3576Google Scholar
- Hull R, Sadler J, Longstaff M (1986) The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. EMBO J 5:3083–3090Google Scholar
- Mann HB, Whitney DR (1947) On a test of whether one of two random variables is stochastically larger than the other. Ann Math Statist 18:50–60Google Scholar
- Rich A (1985) Left-handed Z-DNA and chromatin. In: Marks PA (ed) Genetics, cell differentiation and cancer. Academic Press, New York, pp 25–45Google Scholar
- Richins RD, Scholthof HB, Sheperd RJ (1987) Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res 20:8451–8465Google Scholar
- Rollo F, Covey SN (1985) Cauliflower mosaic virus DNA persists as supercoiled forms in cultured turnip cells. J Gen Virol 66:603–608Google Scholar
- Saenger W (1984) Principles of nucleic acid structure. Springer-Verlag, New YorkGoogle Scholar