Netherlands Journal of Plant Pathology

, Volume 70, Issue 1, pp 18–26 | Cite as

Production of virus-free carnations by means of meristem culture

  • H. van Os


The carnations which are normally grown are practically all infected with various viruses, and therefore attempts were made to obtain virus-free plants of a number of varieties by means of heat treatment and meristem culture. Incubators were designed in which the plants could be subjected to a heat treatment at a temperature of 37°C and at a high degree of humidity of the air. The ultimate percentage of virus-free plants in 1960 amounted to an average of 1.3%. This is sufficient to obtain a virus-free clone, but not enough to build up a virus-free assortment within a short time. The factors which may affect the percentage of successful isolations are discussed, viz. the effect of the season, of varietal differences and of differences between the material supplied by different growers. When the virus-free plants are propagated in practice it is essential that the material should be closely inspected and tested at regular intervals. The new material was found to possess most favourable qualities. It is sold with a special certificate.


Heat Treatment Plant Pathology Varietal Difference Successful Isolation Meristem Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Belgraver, W., — 1950, 1951, 1952. Opbrengstvergelijking van viruszieke anjers. Jaarversl. Proefst. Bloemist. Nederland, Aalsmeer.Google Scholar
  2. Hakkaart, F. A., — 1963. Invloed van enkele virussen op kwaliteit en produktie van anjers. Neth. J. Plant Path. 69: 151–152.Google Scholar
  3. Kassanis, B., — 1955. Some properties of four viruses isolated from carnation plants. Ann. appl. Biol. 40: 103–113.Google Scholar
  4. Limasset, P. &P. Cornuet, — 1949. Recherche de virus de la mosaique du tabac (Marmor tabaci, Holmes) dans les méristèmes des plantes infectées. C. R. Acad. Sci., Paris 228: 1971–1972.Google Scholar
  5. Morel, G. &C. Martin, — 1952. Guérison de dahlias atteints d'une maladie à virus. C. R. Acad. Sci., Paris 235: 1324–1325.Google Scholar
  6. Quak, F., — 1957. Meristeemcultuur, gecombineerd met warmtebehandeling, voor het verkrijgen van virusvrije anjerplanten. Tijdschr. PlZiekt. 63: 13–14.Google Scholar
  7. Quak, F., — 1961. Heat treatment and substances inhibiting virus multiplication, in meristem culture to obtain virus-free plants. Proc. 15th Internat. Congr., Nice, 1958.Google Scholar
  8. Slogteren, D. H. M. van, — 1954. Serological analysis of some plant viruses with the geldiffusion method. Proc. Sec. Conf. Potato Virus Dis., Lisse-Wageningen, 1954: 45–50.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1967

Authors and Affiliations

  • H. van Os
    • 1
  1. 1.Plant Protection Service (P.D.)Wageningen

Personalised recommendations