Journal of Molecular Evolution

, Volume 27, Issue 4, pp 351–364 | Cite as

Evolution of the EF-hand calcium-binding protein family: Evidence for exon shuffling and intron insertion

  • Christine Perret
  • Nourredine Lomri
  • Monique Thomasset


The evolutionary history of the intracellular calcium-binding protein superfamily is well documented. The members of this gene family are all believed to be derived from a common ancestor, which, itself, was the product of two successive gene duplications. In this study, we have compared and analyzed the structures of the recently described genes coding for these proteins. We propose a series of evolutionary events, which include exon shuffling and intron insertion, that could account for the evolutionary origin of all the members of this super-family. According to this hypothesis, the ancestral gene, a product of two successive duplications, consisted of at least four exons. Each exon coding for a peptide (a calcium-binding domain) was separated by an intron that had mediated the duplication. Each distinct lineage evolved from this ancestor by genomic rearrangement, with insertion of introns being a prominent feature.

Key words

Calcium-binding protein EF-hand Exon shuffling Duplication Intron insertion 


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Copyright information

© Springer-Verlag New York Inc 1988

Authors and Affiliations

  • Christine Perret
    • 1
  • Nourredine Lomri
    • 1
  • Monique Thomasset
    • 1
  1. 1.INSERM Unité 120Le VésinetFrance

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