Nonrandomness in prebiotic peptide synthesis
- 89 Downloads
We have synthesized and studied the properties of phosphoanhydrides of alanine with guanosine monophosphate, uridine monophosphate, and adenosine monophosphate. This series of compounds allowed us to investigate the specificity of peptide bond formation in a reaction that could have taken place on the prebiotic earth. We asked whether the intrinsic reactivity of the amino acids, the nature of the nucleotide in the anhydride, or the complementary polynucleotide template influences the specificity of the peptide synthesis reaction. We observed that the differential reactivity of the amino acids results in nearest-neighbor preferences during the peptide synthesis, whereas the nature of the nucleotides and the presence of complementary polynucleotides had no influence on the specificity. These results suggest that some peptides would have been more abundant than others on the prebiotic earth and have implications for the study of the origins of the genetic code and protein synthesis.
Key wordsAmino acyl nucleotide anhydrides Specificity in prebiotic peptides Origin of the genetic code Origin of protein synthesis Template effects
Unable to display preview. Download preview PDF.
- Cairns-smith AG (1982) The genetic takeover and mineral origins of life. Cambridge University Press, CambridgeGoogle Scholar
- Dyson F (1985) Origin of life. Cambridge University Press, CambridgeGoogle Scholar
- Katchalsky A, Ailam G (1967) Polycondensation of amino acid phosphoanhydrides, I. Theoretical. Biochim Biophys Acta 140: 1–13Google Scholar
- Lewinsohn R, Paecht-Horowitz M, Katchalsky A (1967) Polycondensation of amino acid phosphoanhydrides, III. Polycondensation of alanyl adenylate. Biochim Biophys Acta 140: 24–36Google Scholar
- Lipmann F, Tuttle LC (1945) A specific micromethod for the determination of acyl phosphate. J Biol Chem 159:21–28Google Scholar
- Mullins DW, Lacey JC Jr (1981) Factors influencing the rate of non-enzymatic activation of carboxalic and amino acids by ATP. In: Wolman Y (ed) Origin of life. Rediel Publishing, London, pp 209–215Google Scholar
- Nakashima T, Jungck JR, Fox SW, Lederer E, Das BC (1977) A test for randomness in peptides isolated from a thermal polyamino acid. Int J Quantum Chem, Quantum Biol Symp 4:65–72Google Scholar
- Paecht-Horowitz M, Katchalsky A (1967) Polycondensation of amino acid phosphoanhydrides. II. Polymerization of phosphoanhydrides of proline adenylates at constant concentration. Biochim Biophys Acta 140:14–23Google Scholar
- Senaratne N (1986) Direct interactions between amino acids and nucleotides as a possible explanation for the origin of genetic code. PhD dissertation, University of Maryland, College ParkGoogle Scholar
- Tyagi S (1987) An inquiry into prebiotic peptide synthesis. PhD dissertation, University of Maryland, College ParkGoogle Scholar
- Tyagi S, Ponnamperuma C (1990) A study of peptide synthesis by amino acyl nucleotide anhydrides in the presence of complementary homopolynucleotides. In: Eirich F, Ponnamperuma C (eds) Prebiological self-organization of matter. A. Deepak Publishing, Hampton, Virginia, pp 197–210Google Scholar