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Evolution of the autosomal chorion cluster inDrosophila. II. Chorion gene expression and sequence comparisons of thes16 ands19 genes in evolutionarily distant species

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Summary

We present a total of 13 kb of DNA sequences, encompassing autosomal chorion genes and their flanking DNA in four species of the genusDrosophila. Against a background of extensive divergence in introns and even in parts of the coding regions, islands of strong conservation are evident in the proximal 5′ flanking and 5′ untranslated sequences. An extragenic region of strong conservation is seen downstream of the last chorion gene in the autosomal cluster. The conserved DNA elements may be related to the conserved regulatory features of this cluster, including gene amplification and tissue- and temporally regulated transcription.

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References

  • Beverley SM, Wilson AC (1984) Molecular evolution inDrosophila and the higher diptera. II. A time scale for fly evolution. J Mol Evol 21:1–13

    PubMed  Google Scholar 

  • Biggen MD, Gibson TJ, Hong GF (1983) Buffer-gradient gels and35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80:3963–3965

    PubMed  Google Scholar 

  • Choi O-R, Engel JD (1986) A 3′ enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult β-globin gene. Nature 323:731–734

    Article  PubMed  Google Scholar 

  • Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995

    PubMed  Google Scholar 

  • Dale DMK, McClure BA, Houchins JP (1985) A rapid singlestranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing to corn mitochondrial 18S rDNA. Plasmid 13: 31–40

    Article  PubMed  Google Scholar 

  • Delidakis C, Kafatos FC (1987) Amplification of a chorion gene cluster inDrosophila is subject to multiplecis-regulatory elecments and to long-range position effects. J Mol Biol 197: 11–26

    Article  PubMed  Google Scholar 

  • Delidakis C, Kafatos FC (1989) Replication origins and multiplecis-acting elements for normal amplification of the autosomal chorion genes inDrosophila. EMBO J 8:891–901

    PubMed  Google Scholar 

  • Griffin-Shea R, Thireos G, Kafatos FC (1982) Organization of a cluster of four chorion genes inDrosophila and its relationship to developmental expression and amplification. Dev Biol 91:325–336

    Article  PubMed  Google Scholar 

  • King RC (1970) Ovarian development inDrosophila melanogaster. Academic Press, New York

    Google Scholar 

  • Laughon A, Scott MP (1984) Sequence of aDrosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature 310:25–31

    Article  PubMed  Google Scholar 

  • Levine J, Spradling AD (1985) DNA sequence of a 3.8 kilobase pair region controllingDrosophila chorion gene amplification. Chromosoma 92:136–142

    Article  PubMed  Google Scholar 

  • Levinson A, Silver D, Seed B (1984) Minimal size plasmids containing an m13 origin for production of single-strand transducing particles. J Mol Appl Genet 2:507–517

    PubMed  Google Scholar 

  • Margaritis LH (1986) The eggshell ofDrosophila melanogaster. II. New staging characteristics and fine structural analysis of choriogenesis. Can J Zool 64:2152–2175

    Google Scholar 

  • Mariani BD, Lingappa JR, Kafatos FC (1988) Temporal regulation in development: negative and positive cis regulators dictate the precise timing of expression of aDrosophila chorion gene. Proc Natl Acad Sci USA 85:3029–3033

    PubMed  Google Scholar 

  • Martínez-Cruzado JC (1988) Evolution of the organization and DNA sequence of the autosomal chorion gene cluster in HawaiianDrosophila. Thesis, Harvard University, Cambridge MA

    Google Scholar 

  • Martínez-Cruzado JC, Swimmer C, Fenerjian MG, Kafatos FC (1988) Evolution of the autosomal chorion locus inDrosophila. I. General organization of the locus and sequence comparisons of genes s15 and s19 in evolutionarily distant species. Genetics 119:663–677

    PubMed  Google Scholar 

  • Messing J (1983) New m 13 vectors for cloning. Meth Enzymol 101:20–78

    PubMed  Google Scholar 

  • Mount SM (1982) A catalogue of splice junction sequences. Nucleic Acids Res 10:459–472

    PubMed  Google Scholar 

  • Orr W, Komitopoulou K, Kafatos FC (1984) Mutants suppressing in trans chorion gene amplification inDrosophila. Proc Natl Acad Sci USA 81:3773–3777

    PubMed  Google Scholar 

  • Orr-Weaver TL, Spradling AC (1986)Drosophila chorion gene amplification requires an upstream region regulating s 18 transcription. Mol Cell Biol 6:4624–4633

    PubMed  Google Scholar 

  • Parks S, Spradling A (1987) Spatially regulated expression of chorion genes duringDrosophila oogenesis. Genes & Dev 1: 497–509

    Google Scholar 

  • Parks S, Wakimoto B, Spradling A (1986) Replication and expression of an X-linked cluster ofDrosophila chorion genes. Dev Biol 117:294–305

    Article  PubMed  Google Scholar 

  • Pustell J, Kafatos FC (1982) A high speed, high capacity homology matrix: zooming through SV40 and polyoma. Nucleic Acids Res 10:4765–4782

    PubMed  Google Scholar 

  • Pustell J, Kafatos FC (1984) A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Res 12:643–655

    PubMed  Google Scholar 

  • Romano CP, Bienz-Tadmor B, Mariani BD, Kafatos FC (1988) Both early and lateDrosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene. EMBO J 7:783–790

    PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    PubMed  Google Scholar 

  • Snyder M, Hunkapiller M, Yuen D, Silvert D, Fristrom J, Davidson N (1982) Cuticle protein genes ofDrosophila: structure, organization and evolution of four clustered genes. Cell 29:1027–1040

    Article  PubMed  Google Scholar 

  • Spradling AC (1981) The organization and amplification of two chromosomal domains containingDrosophila chorion genes. Cell 27:193–201

    Article  PubMed  Google Scholar 

  • Spradling AC, Mahowald AP (1980) Amplification of genes for chorion proteins during oogenesis inDrosophila melanogaster. Proc Natl Acad Sci USA 77:1096–1100

    PubMed  Google Scholar 

  • Spradling AC, DeCicco DV, Wakimoto BT, Levine JF, Kalfayan LJ, Cooley L (1987) Amplification of the X-linkedDrosophila chorion gene cluster requires a region upstream from the s38 chorion gene. EMBO J 6:1045–1053

    PubMed  Google Scholar 

  • Staden R (1982) Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res 10:4731–4751

    PubMed  Google Scholar 

  • Staden R (1984) A computer program to enter DNA gel reading data into a computer. Nucleic Acids Res 12:499–504

    PubMed  Google Scholar 

  • Tabor S, Richardson CC (1987) DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci USA 84:4767–4771

    PubMed  Google Scholar 

  • Thireos G, Griffin-Shea R, Kafatos FC (1980) Untranslated mRNA for a chorion protein inDrosophila melanogaster accumulates transiently at the onset of specific gene amplification. Proc Natl Acad Sci USA 77:5789–5793

    PubMed  Google Scholar 

  • Throckmorton LH (1975) The phylogeny, ecology and geography ofDrosophila. In: King RC (ed) Handbook of genetics, vol 3. Plenum Press, New York, pp 421–469

    Google Scholar 

  • Wong Y-C, Pustell J, Spoerel N, Kafatos FC (1985) Coding and potential regulatory sequences of a cluster of chorion genes inDrosophila melanogaster. Chromosoma 92:124–135

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, 16 Divinity Avenue, 02138, Cambridge, Massachusetts, USA

    Maryanne G. Fenerjian, Juan Carlos Martínez-Cruzado, Candace Swimmer, Dennis King & Fotis C. Kafatos

  2. Institute of Molecular Biology and Biotechnology, Research Center of Crete, PO Box 1527, 71110, Heraklio, Crete, Greece

    Fotis C. Kafatos

Authors
  1. Maryanne G. Fenerjian
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  2. Juan Carlos Martínez-Cruzado
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  3. Candace Swimmer
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  4. Dennis King
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  5. Fotis C. Kafatos
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Fenerjian, M.G., Martínez-Cruzado, J.C., Swimmer, C. et al. Evolution of the autosomal chorion cluster inDrosophila. II. Chorion gene expression and sequence comparisons of thes16 ands19 genes in evolutionarily distant species. J Mol Evol 29, 108–125 (1989). https://doi.org/10.1007/BF02100110

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  • DOI: https://doi.org/10.1007/BF02100110

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