Summary
The condensation reactions of activated nucleotides, ImpN or 2-MeImpN, with the selfcomplementary ribo-octanucleotide GCGCGCGC or with the partially self-complementary heptanucleotide GCGCGCG were studied. The templatedirected reaction of 2-meImpC with the heptamer yields the 3′–5′ octamer as the main product. All other reactions yield 2′–5′-linked octamers and pyrophosphates as major products. Surprisingly, 2-MeImpG facilitates the reaction of 2-MeImpC with the heptamer.
Procedures for the analysis by gel electrophoresis of the oligomeric products obtained in reactions of this kind are described.
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Abbreviations
- A:
-
adenosine
- C:
-
cytidine
- G:
-
guanosine
- U:
-
uridine
- ImpN (N=A, C, G, or U):
-
nucleoside 5′-phosphorimidazolide
- 2-MeImpN (N=A, C, G, or U):
-
nucleoside 5′-phospho-2-methylimidazolide
- 2-MeImpGpC:
-
3′–5′-linked dinucleoside-5′-phosphos-2-methylimidazolide
- N2′p5′N:
-
a dinucleotide with a 2′–5′ internucleotide linkage. An expression such as GCGCG denotes an oligonucleotide with a 5′-terminal phosphate and with all 3′–5′ internucleotide phosphodiester linkages
References
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Ng, Km.E., Orgel, L.E. Reaction on alternating GC oligonucleotide templates. J Mol Evol 29, 101–107 (1989). https://doi.org/10.1007/BF02100109
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DOI: https://doi.org/10.1007/BF02100109