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Journal of Molecular Evolution

, Volume 24, Issue 1–2, pp 53–60 | Cite as

Concerted evolution of sequence repeats inDrosophila mitochondrial DNA

  • Michel Solignac
  • Monique Monerot
  • Jean-Claude Mounolou
Article

Summary

In the eightDrosophila species of themelanogaster subgroup, the mitochondrial DNA (mtDNA) contains an A+T-rich region in which replication originates. The length of this region, in contrast with that of the coding part of the genome, varies extensively among these species. The A+T-rich region ranges from about 1kbp inD. yakuba, D. teissieri, D. erecta, andD. orena to 5 kbp inD. melanogaster, D. simulans, D. mauritiana, andD. sechellia. The difference in size is due in part to the amplification, in the species with long genomes, of a 470-bp sequence that is present only once in each of the four species with short genomes.

Usually three to six repeats of this sequence occur in direct tandem repetition in the species with long genomes. The sequence is characterized by the relative positions of the Hpa I and Acc I cleavage sites. Comparative study of the genomes found in the species with long mtDNA molecules reveals relative homogeneity of the repeat units within a given genome, which contrasts with the variability found among the repeats of different genomes. This result is suggestive of a process of a concerted evolution.

The examination of heteroplasmic flies of three species (D. simulans, D. mauritiana, andD. sechellia) has shed light on this process. In most cases the molecular types of mtDNA present in a heteroplasmic individual differ by one repeat unit. Addition or deletion of this sequence appears to be the original mutational event generating transient heteroplasmy. Cycles of addition or deletion may consequently maintain the intragenomic homogeneity of the repeats.

Finally, we have analyzed an exceptional isofemale line in which three molecular lengths of mtDNA are found (molecules with four, five, and six repeats, respectively). Individual offspring of this line carry from one to three of the molecular types, in all combinations. This indicates that the remodeling of the mitochondrial genome occurs through a mechanism that is at present unknown, but that is site specific and rather frequent.

Key words

Drosophila melanogaster subgroup Mitochondrial DNA A+T-rich region Length variation Concerted evolution Triplasmy 

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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Michel Solignac
    • 1
  • Monique Monerot
    • 2
  • Jean-Claude Mounolou
    • 2
  1. 1.Laboratoire de Biologie et Génétique EvolutivesCentre National de la Recherche ScientifiqueGif-sur-YvetteFrance
  2. 2.Laboratoire de Biologie GénéraleUniversité Paris XIOrsay CedexFrance

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