Journal of Molecular Evolution

, Volume 23, Issue 2, pp 108–112 | Cite as

The template properties of some oligodeoxynucleotides containing cytidine and guanosine

  • Tomasz Haertle
  • Leslie E. Orgel


the co-condensation of guanosine- and cytidine-5′-phospho-2-methylimidazolide on various oligodeoxynucleotides containing C and G has been studied. We find that GC7 is an effective template for the incorporation of C into products of the form GnC, whereas C7G does not act as a template for C incorporation. The template C3GC3GC3GC3 directs the synthesis of complementary products, but the yield of long oligomers is very small. Templates in which G residues are contiguous or separated by a single C residue are ineffective, while templates containing the sequence GCCG are very inefficient. The significance of these findings in the context of prebiotic chemistry is discussed.

Key words

Template synthesis Nucleotide oligomerization Prebiotic 







cytidine-5′-monophosphoric acid; poly(C), polycytidylic acid


an oligonucleotide with composition GnC and terminated by a 5′-phosphate


an oligonucleotide with the sequence GiCGj and terminated by a 5′-phosphate


high-pressure liquid chromatography


ultraviolet light


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chen CB, Inoue T, Orgel LE (1985) Template-directed synthesis on oligodeoxycytidylate and polydeoxycytidylate templates. J Mol Biol 181:271–279CrossRefPubMedGoogle Scholar
  2. Chu BCF, Orgel LE (1985) Nonenzymatic sequence-specific cleavage of single-stranded DNA. Proc Natl Acad Sci USA 82:963–967PubMedGoogle Scholar
  3. Haertle T, Orgel LE (1986) Template-directed synthesis on the oligonucleotide d(C7GC7). J Mol Biol 188:72–80CrossRefGoogle Scholar
  4. Inoue T, Orgel LE (1982) Oligomerization of (guanosine 5′-phosphor)-2-methylimidazolide on poly(C): an RNA polymerase model. J Mol Biol 162:201–217CrossRefPubMedGoogle Scholar
  5. Inoue T, Joyce GF, Grzeskowiak K, Orgel LE, Brown JM, Reese C (1984) Template-directed synthesis on the pentanucleotide CpCpGpCpC. J Mol Biol 178:669–676CrossRefPubMedGoogle Scholar
  6. Joyce GF, Inoue T, Orgel LE (1984) Non-enzymatic template-directed synthesis on RNA random copolymers. J Mol Biol 176:279–306CrossRefPubMedGoogle Scholar
  7. Joyce GF, Orgel LE (1986) Non-enzymatic template-directed synthesis on RNA random copolymers: poly(C, G) templates. J. Mol Biol, in pressGoogle Scholar
  8. Lo K-M, Jones SS, Hackett NR, Khorana HG (1984) Specific amino acid substitutions in bacterioopsin: replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons. Proc Natl Acad Sci USA 81:2285–2289Google Scholar
  9. Matteucci MD, Caruthers MH (1981) Synthesis of deoxyoligonucleotides on a polymer support. J Am Chem Soc 103:3185–3191CrossRefGoogle Scholar
  10. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Tomasz Haertle
    • 1
  • Leslie E. Orgel
    • 1
  1. 1.The Salk Institute for Biological StudiesSan DiegoUSA

Personalised recommendations