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Journal of Molecular Evolution

, Volume 23, Issue 2, pp 108–112 | Cite as

The template properties of some oligodeoxynucleotides containing cytidine and guanosine

  • Tomasz Haertle
  • Leslie E. Orgel
Article

Summary

the co-condensation of guanosine- and cytidine-5′-phospho-2-methylimidazolide on various oligodeoxynucleotides containing C and G has been studied. We find that GC7 is an effective template for the incorporation of C into products of the form GnC, whereas C7G does not act as a template for C incorporation. The template C3GC3GC3GC3 directs the synthesis of complementary products, but the yield of long oligomers is very small. Templates in which G residues are contiguous or separated by a single C residue are ineffective, while templates containing the sequence GCCG are very inefficient. The significance of these findings in the context of prebiotic chemistry is discussed.

Key words

Template synthesis Nucleotide oligomerization Prebiotic 

Abbrevations

2-MeImpC

cytidine-5′-phospho-2-methylimidazolide

2-MeImpG

guanosine-5′-phospho-2-methylimidazolide

pC

cytidine-5′-monophosphoric acid; poly(C), polycytidylic acid

GnC

an oligonucleotide with composition GnC and terminated by a 5′-phosphate

(pG)ipC(pG)j

an oligonucleotide with the sequence GiCGj and terminated by a 5′-phosphate

HPLC

high-pressure liquid chromatography

UV

ultraviolet light

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References

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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Tomasz Haertle
    • 1
  • Leslie E. Orgel
    • 1
  1. 1.The Salk Institute for Biological StudiesSan DiegoUSA

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