The template properties of some oligodeoxynucleotides containing cytidine and guanosine
the co-condensation of guanosine- and cytidine-5′-phospho-2-methylimidazolide on various oligodeoxynucleotides containing C and G has been studied. We find that GC7 is an effective template for the incorporation of C into products of the form GnC, whereas C7G does not act as a template for C incorporation. The template C3GC3GC3GC3 directs the synthesis of complementary products, but the yield of long oligomers is very small. Templates in which G residues are contiguous or separated by a single C residue are ineffective, while templates containing the sequence GCCG are very inefficient. The significance of these findings in the context of prebiotic chemistry is discussed.
Key wordsTemplate synthesis Nucleotide oligomerization Prebiotic
cytidine-5′-monophosphoric acid; poly(C), polycytidylic acid
an oligonucleotide with composition GnC and terminated by a 5′-phosphate
an oligonucleotide with the sequence GiCGj and terminated by a 5′-phosphate
high-pressure liquid chromatography
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