Journal of tissue culture methods

, Volume 8, Issue 2, pp 79–81 | Cite as

Preparation of brain cell cultures of mice

  • Malcolm S. Trueblood


A procedure for an in vitro technique used to establish cells in culture from the brain of the mouse is described. The success of establishing cell cultures is dependent upon the dissociation medium used, the concentration of fetal bovine serum, and the site of the brain removed for culture. Extreme care must be observed in establishing adherence of the cells to the plastic flasks used in the procedure. Dissociated cultures are found to be superior to explant cultures in establishing cell growth.

Key words

brain collagenase mouse primary culture 


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Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • Malcolm S. Trueblood
    • 1
  1. 1.Division of Microbiology and Veterinary MedicineUniversity of WyomingLaramieUSA

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