Journal of tissue culture methods

, Volume 8, Issue 2, pp 79–81 | Cite as

Preparation of brain cell cultures of mice

  • Malcolm S. Trueblood
Article
  • 425 Downloads

Summary

A procedure for an in vitro technique used to establish cells in culture from the brain of the mouse is described. The success of establishing cell cultures is dependent upon the dissociation medium used, the concentration of fetal bovine serum, and the site of the brain removed for culture. Extreme care must be observed in establishing adherence of the cells to the plastic flasks used in the procedure. Dissociated cultures are found to be superior to explant cultures in establishing cell growth.

Key words

brain collagenase mouse primary culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gilden, D. H.; Devlin, M.; Wroblewska, Z.; Friedman, H.; Rorke, L. B.; Sontoli, D.; Koprowski, H. Human brain in tissue culture. J. Comp. Neurol. 161: 295–306; 1975.PubMedGoogle Scholar
  2. 2.
    Phillips, H. J. Dye exclusion tests for cell viability. In: Kruse, P. F., Jr.; Patterson, M. K., Jr., eds. Tissue culture: Methods and applications. New York: Academic Press; 1973: 406.Google Scholar
  3. 3.
    Schmidt, N. J. Cell culture techniques for diagnostic virology. In: Jenette, E. H.; Schmidt, H. J., eds. Diagnostic procedures for viral, rickettsial and chlamydial infections. Washington, D.C.: Am. Public Health Assoc.; 1979: 79.Google Scholar

Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • Malcolm S. Trueblood
    • 1
  1. 1.Division of Microbiology and Veterinary MedicineUniversity of WyomingLaramieUSA

Personalised recommendations