Preparation of brain cell cultures of mice
- 425 Downloads
A procedure for an in vitro technique used to establish cells in culture from the brain of the mouse is described. The success of establishing cell cultures is dependent upon the dissociation medium used, the concentration of fetal bovine serum, and the site of the brain removed for culture. Extreme care must be observed in establishing adherence of the cells to the plastic flasks used in the procedure. Dissociated cultures are found to be superior to explant cultures in establishing cell growth.
Key wordsbrain collagenase mouse primary culture
Unable to display preview. Download preview PDF.
- 2.Phillips, H. J. Dye exclusion tests for cell viability. In: Kruse, P. F., Jr.; Patterson, M. K., Jr., eds. Tissue culture: Methods and applications. New York: Academic Press; 1973: 406.Google Scholar
- 3.Schmidt, N. J. Cell culture techniques for diagnostic virology. In: Jenette, E. H.; Schmidt, H. J., eds. Diagnostic procedures for viral, rickettsial and chlamydial infections. Washington, D.C.: Am. Public Health Assoc.; 1979: 79.Google Scholar