Journal of tissue culture methods

, Volume 8, Issue 2, pp 53–58 | Cite as

Culture of alveolar epithelial Type II cells isolated from adult rat lung

  • Robert D. Greenleaf


A method is described for isolating alveolar Type II cells from adult rat lung and subsequent primary culture of the cells. Certain potential problems that might arise during the procedure are elucidated and means of minimizing their affect on the procedure's success are discussed.

Key words

lung Type II cells isolation primary culture 


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Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • Robert D. Greenleaf
    • 1
  1. 1.Department of Anatomy and Cellular BiologyTufts University School of MedicineBostonUSA

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