Regional differences in local cerebral blood flow (LCBF) and glucose utilization (LCGU) in the basal ganglia after occlusion of the middle cerebral artery in rats
Acute effects of occlusion of the middle cerebral artery on local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) were investigated quantitatively in separate groups of rats using (14C) iodoantipyrine (14C-IAP) or (14C) 2-deoxyglucose (14C-DG) respectively.
LCBF was significantly decreased in the ipsilateral cerebral cortices (to less than 45 ml/100 g/min or 30% of the control side) and the lateral part of the striatum (to 22 ml/100 g/min or 10% of the control side) which were supplied by the middle cerebral artery. No significant changes in LCBF were found in any other of the subcortical regions. In contrast to the unanimous decrease of LCBF in the ipsilateral cortices and the lateral striatum, complexed changes in LCGU were found in not only the cortex and striatum but also in many other subcortical regions which were closely related to the distribution of the mesencephalic dopamine neurons, such as globus pallidus, substantia nigra, subthalamic nucleus, nucleus accumbens, olfactory tubercle and lateral habenular nucleus. Relevance of this putative neurotransmitter and GABA on the glucose metabolism in ischemic brain is discussed.
Key wordsCerebral ischemia cerebral blood flow cerebral glucose utilization basal ganglia dopamine
local cerebral glucose utilization
local cerebral blood flow
middle cerebral artery
New England Nuclear Co.
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- Ginsberg MD, Reivich M, Giandomenico A, Greenberg JH (1977) Local glucose utilization in acute focal cerebral ischemia: local dysmetabolism and diaschisis. Neurology (Minneap) 27: 1042–1048Google Scholar
- Ginsberg MD, Reivich M (1979) Use of the 2-deoxyglucose method of local cerebral glucose utilization in the abnormal rat brain: evaluation of the lumped constant during ischemia. Acta Neurol Scand [Suppl] 60(72): 226–227Google Scholar
- Gjedde A, Wienhard K, Heiss W-D, Kloster G, Diemer NH, Herholz K, Pawlik G (1985) Comparative regional analysis of 2-fluorodeoxyglucose and methylglucose uptake in brain of four stroke patients. With special reference to the regional estimation of the lumped constant. J Cereb Blood Flow Metab 5: 163–178PubMedGoogle Scholar
- Kelly PAT, McCulloch J (1981) Cerebral blood flow and glucose utilization following dopaminergic and GABAergic manipulation. J Cereb Blood Flow Metab, vol 1 [Suppl] 1: 317–318Google Scholar
- McCulloch J, Kelly PAT, Crome JJ (1981) GABAergic influence upon cerebral metabolism and perfusion. In: Cervos-Navarro J, Fritschka E (eds) Cerebral microcirculation and metabolism. Raven Press, New York, pp 189–193Google Scholar
- Pellegrino LJ, Cushman AJ (1968) A stereotaxic atlas of the rat brain. Appleton-Century-Crofts, New YorkGoogle Scholar
- Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with (14C) iodoantipyrine. Am J Physiol 234: H 59–66Google Scholar
- Sokoloff L, Reivich M, Kennedy C, DesRosiers MH, Patlak CS, Petigrew KD, Sakurada O, Shinohara M (1977) (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rats. J Neurochem 28: 897–916PubMedGoogle Scholar
- Tamura A, Graham DI, McCulloch J, Teasdale GW (1981 a) Focal cerebral ischemia in the rat: 1. Description of technique and early neurological consequences following middle cerebral artery occlusion. J Cereb Blood Flow Mctab 1: 53–60Google Scholar