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Detection of JC virus by polymerase chain reaction in cerebrospinal fluid from two patients with progressive multifocal leukoencephalopathy

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Abstract

The polymerase chain reaction (PCR) was used to identify JC virus (JCV) in the cerebrospinal fluid of two patients with progressive multifocal leukoencephalopathy confirmed by brain biopsy. In addition, JCV viremia was demonstrated by PCR in one case. JCV detection in spinal fluid by PCR may be the first non-invasive technique available for the diagnostic confirmation of progressive multifocal leukoencephalopathy.

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References

  1. Padgett BL, Walker DL, Zurheim GM, Eckroade RJ, Dessel BH: Cultivation of papova-like viruses from human brain with progressive multifocal leukoencephalopathy. Lancet 1971, i: 1257–1260.

    Article  Google Scholar 

  2. Walker DL, Frisque RJ: The biology and molecular biology of JC virus. In: Salzman ND (ed): The papovaviridae. Plenum Press, New York 1986, p. 327–377.

    Google Scholar 

  3. Richardson EP: Progressive multifocal leukoencephalopathy. New England Journal of Medicine 1961, 265: 815–823.

    PubMed  Google Scholar 

  4. Aksamit AJ, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: JC virus detection by in situ hybridization compared with immunocytochemistry. Neurology 1986, 36: 499–504.

    PubMed  Google Scholar 

  5. Aksamit AJ, Major EO, Ghatak NR, Sidhu GS, Parisi JE, Guccion JG: Diagnosis of progressive multifocal leukoencephalopathy by brain biopsy with biotinlabeled DNA:DNA in situ hybridization. Journal of Neuropathology and Experimental Neurology 1987, 46: 556–566.

    PubMed  Google Scholar 

  6. Telenti A, Aksamit AJ, Proper J, Smith TF: Detection of JC virus DNA by polymerase chain reaction in patients with progressive multifocal leukoencephalopathy. Journal of Infectious Diseases 1990, 162: 858–861.

    PubMed  Google Scholar 

  7. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Annals of Neurology 1985, 18: 490–496.

    Article  PubMed  Google Scholar 

  8. Wolinsky SM, Rinaldo CR, Kwok S, Sninsky JJ, Gupta P, Imagawa D, Farzadegan H, Jacobson LP, Grovit KS, Lee MH, Chmiel JS, Ginzburg H, Kaslow RA, Phair JP: Human immunodeficiency virus type 1 infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men. Annals of Internal Medicine 1989, 111: 961–972.

    PubMed  Google Scholar 

  9. Arthur RR, Dagostin S, Shah KV: Detection of BK and JC virus in urine and brain tissue by the polymerase chain reaction. Journal of Clinical Microbiology 1989, 27: 1174–1179.

    PubMed  Google Scholar 

  10. Rowley AH, Whitley RJ, Lakeman FD, Wolinsky SM: Rapid detection of Herpes simplex virus DNA in cerebrospinal fluid of patients with Herpes simplex encephalitis. Lancet 1990, i: 440–441.

    Article  Google Scholar 

  11. Shaunak S, Albright RE, Klotman ME, Henry SC, Bartlett JA, Hamilton JD: Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurological disease. Journal of Infectious Diseases 1990, 161: 1068–1072.

    PubMed  Google Scholar 

  12. Chester PM, Heritage J, McCance DJ: Persistence of DNA sequences of BK and JC virus in normal human tissues. Journal of Infectious Diseases 1983, 147: 676–684.

    PubMed  Google Scholar 

  13. Houff SA, Major EO, Katz DA, Kufta CV, Sever JL, Pittaluga S, Roberts JR, Gitt J, Saini N, Lux W: Involvement of JC virus-infected mononuclear cells from the bone marrow and spleen in the pathogenesis of progressive multifocal leukoencephalopathy. New England Journal of Medicine 1988, 318: 301–305.

    PubMed  Google Scholar 

  14. Marshall WF, Telenti A, Proper J, Aksamit AJ, Smith TF: Survey of urine from transplant recipients for polyomavirus JC and BK using the polymerase chain reaction. Molecular and Cellular Probes 1991, 5: 125–128.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Medical Microbiology, University of Berne, Friedbuehlstrasse 51, 3010, Berne, Switzerland

    A. Telenti

  2. Division of Infectious Disease, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA

    A. Telenti, W. F. Marshall & J. D. Smilack

  3. Department of Neurology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA

    A. J. Aksamit

  4. Section of Clinical Microbiology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA

    T. F. Smith

Authors
  1. A. Telenti
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  2. W. F. Marshall
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  3. A. J. Aksamit
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  4. J. D. Smilack
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  5. T. F. Smith
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Telenti, A., Marshall, W.F., Aksamit, A.J. et al. Detection of JC virus by polymerase chain reaction in cerebrospinal fluid from two patients with progressive multifocal leukoencephalopathy. Eur. J. Clin. Microbiol. Infect. Dis. 11, 253–254 (1992). https://doi.org/10.1007/BF02098091

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  • DOI: https://doi.org/10.1007/BF02098091

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