Skip to main content
SpringerLink
Log in

Einfluß von Aminosäuren auf das Wachstumin vitro kultivierter Wurzeln vonPisum sativum L.

Effects of amino acids on the growth of pea roots (Pisum sativum L.) cultivatedin vitro

Влияние аминокислот на рост корешковPisum sativum L., культивируемыхin vitro

  • Originalarbeiten
  • Published:
Die Kulturpflanze Aims and scope Submit manuscript

Zusammenfassung

Zur Vorbereitung von Untersuchungen über den Aminosäure-Stoffwechsel von Erbsenwurzeln wurde der Einfluß zahlreicher Aminosäuren auf das Wachstum invitro kultivierter Erbsenwurzeln geprüft. Isomolare Mengen verschiedener Protein-Aminosäuren beeinflussen das Wachstum mit unterschiedlicher Intensität. Der Grad der Wachstumshemmung ändert sich in Abhängigkeit von der Konzentration bei verschiedenen Aminosäuren in unterschiedlichem Maße. Durch Vergleich der Beeinflussung von Trockengewicht und Längenwachstum der Wurzeln konnte wahrscheinlich gemacht werden, daß es Aminosäuren gibt, die das Wachstum hemmen und zu erheblichen Substanzverlusten führen, weiterhin solche, die auch das Wachstum hemmen, dabei aber keinen verstärkten Katabolismus verursachen und schließlich solche, die gleichfalls das Wachstum hemmen, die Stoffproduktion aber kaum beeinflussen. Zwischen dem Aminosäure-Einfluß auf das Wachstum von Erbsenwurzelkulturen und Tomatenwurzelkulturen (Street et al. 1960) bestehen bemerkenswerte Übereinstimmungen.

Summary

With respect to investigations on amino acid metabolism in pea roots, which we had in view, the effects of numerous amino acids on pea roots cultivated invitro were examined. By isomolare concentrations of different amino acids root growth was found to be influenced with different intensity. The degree of growth inhibition caused by different amino acids varies in relation to amino acid concentration in different proportion. By reason of comparison of dry matter production and longitudinal root growth it seems to be probable that there exist amino acids causing growth inhibition connected with strong substance loss, furthermore, such which also inhibit growth and do not markedly promote catabolic processes, and finally such causing again growth inhibition but without any distinct influence on substance production. Between effects of amino acids in vitro on pea roots and tomato roots (Street et al. 1960) a remarkable correspondence has been stated.

Краткое содержание

Для подготовки исследований по аминкислотному обмену изучалось влияние многочисленных аминокислот на рост корешков гороха, культивируемых invitro. Изомолярные количества различных протеиновых амино-кислот влияют на рост с различной интенсивностью. Степень торможения роста изменяется в зависимости от концентрации различных кислот в различной степени. Путем сравнения силы влияния на сухой вес и на рост растяжения корешков, можно принять, что существуют аминокислоты, которые тормозят рост и приводят к существенной потери вещества; затем такие, которые тоже тормозят рост, но не ведут к усиленному катаболизму; наконец есть и такие, которые только тормозят рост, но почти не влияют на продукцию вещества. Влияние аминокислот на рост культур корешков гороха в значительной степени соответствует влиянию на рост культур корешков томата (Street et al. 1960).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  • Audus, L. J., and J. H. Quastel, 1947: Toxic effects of some amino acids and amines on seedling growth. — Nature, London160, 222–223.

    Google Scholar 

  • Bonner, J., and F. T. Addicott, 1937: Cultivation in vitro of excised pea roots. — Bot. Gaz., Chicago,99, 144–170.

    Google Scholar 

  • Boswell, J. G., 1950: Metabolic systems in the “root” ofBrassica napus L. — Ann. Bot., London, n. Ser.,14, 521–543.

    Google Scholar 

  • Böszörmenyi, Z., and E. Cseh, 1962: The effect of L-amino acids on the uptake of C14-glycine, C14-tyrosine and S35-methionine by excised wheat roots. — Acta biol. Acad. Sci. Hung.8, 39–49.

    Google Scholar 

  • Feketé, M., E. Cseh and B. Böszörmenyi, 1967: Effect of preloading on alanine absorption by wheat roots. — Physiol. Plantarum, Kopenhagen20, 400–407.

    Google Scholar 

  • Fowden, L., 1967: Aspects of amino acid metabolism in plants. — Ann. Rev. Plant Physiol.18, 85–106.

    Google Scholar 

  • Fries, N., 1951: The influence of amino acids on growth and lateral root formation in cotyledonless pea seedlings. — Experientia, Basel7, 378–379.

    Google Scholar 

  • —— 1953: Limiting factors in the growth of the pea seedling root. — Physiol. Plantarum, Kopenhagen6, 292–300.

    Google Scholar 

  • —— 1955: Further experiments on the response of decotylized pea seedlings to arginine. — Physiol. Plantarum, Kopenhagen8, 164–173.

    Google Scholar 

  • Harris, G. P., 1956: Amino acids as sources of nitrogen for the growth of isolated oat embryos. — New Phytol., England55, 253–268.

    Google Scholar 

  • Helanová, I., and Z. Sladky, 1967: The effect of glycine, humus substances and sucrose on the growth of tomato roots in vitro. — Biol. Plantarum, Prag9, 276–284.

    Google Scholar 

  • Holleman, J. M., and J. L. Key, 1967: Inactive and protein precursor pools of amino acids in the soybean hypocotyl. — Plant Physiol., Lancaster42, 29–36.

    Google Scholar 

  • Hubac, C., 1967: Acroissement chez des plantules de la resistance à la dessiccation par action préable de la proline. — C. R. Acad. Sci., Paris Ser. D,264, 1286–1289.

    Google Scholar 

  • Miettinen, J. K., 1957: Uptake of amino acids by the pea-plant (Pisum sativum). Mechanism studied using14C-labelled alanine and glutamic acid (preliminary communication). — Suom. Kemistil. Ser. B,30, 30.

    Google Scholar 

  • Miettinen, J. K., and H. Waris, 1958: A chemical study of the neomorphosis induced by glycine inOenanthe aquatica. — Physiol. Plantarum, Kopenhagen11, 193–199.

    Google Scholar 

  • Miller, R. H., and E. L. Schmidt, 1965: Uptake and assimilation of amino acids supplied to the sterile soil: root environment of the bean plant (Phaseolus vulgaris). — Soil Sci., Baltimore100, 323–330.

    Google Scholar 

  • Oaks, A., 1964: The synthesis of leucine in maize embryos. — Plant Physiol., Lancaster39, Suppl. XIX.

  • —— 1965: The soluble leucine pool in maize root tips. — Plant Physiol., Lancaster40, 142–149.

    Google Scholar 

  • —— 1965: The effect of leucine on the biosynthesis of leucine in maize root tips. — Plant Physiol., Lancaster40, 149–155.

    Google Scholar 

  • —— 1965: The synthesis of leucine in maize embryos. Biochim. biophys. Acta, Amsterdam111, 79–89.

    Google Scholar 

  • —— 1966: Transport of amino acids to maize root. — Plant Physiol., Lancaster41, 173–176.

    Google Scholar 

  • Oaks, A., and H. Beevers, 1964: The requirement for organic nitrogen inZea mays embryos. — Plant Physiol., Lancaster39, 37–43.

    Google Scholar 

  • Polter, C., 1966: Einfluß verschiedener Kulturbedingungen auf Wachstum, Entwicklung, Sauerstoffverbrauch und andere Eigenschaften der Wurzeln vonPisum sativum L. — Kulturpflanze14, 97–127.

    Google Scholar 

  • —— 1967: Einfluß von Penicillin in hoher Konzentration auf Wachstum und Sauerstoffaufnahme von Erbsenwurzeln bei Kultur in vitro. — Kulturpflanze15, 293–309.

    Google Scholar 

  • Ratner, E. I., S. F. Kolosov, S. F. Ukhina, I. N. Dobrochotova and O. N. Kazuto, 1956: Über die Assimilierung von Aminosäuren durch Pflanzen als Stickstoffquelle (russisch). — Izv. Akad. Nauk SSSR, Ser. Biol., Jg. 1956, 64–82.

    Google Scholar 

  • Ratner, E. I., A. M. Smirnow, H. Huan, S. F. Ukhina and I. N. Kuzovkina, 1963: Assimilation of amino acids as a nitrogen source by isolated alfalfa roots and whole pea plants in sterile cultures (russisch, engl. Zusammenfassung). — Fiziol. Rastenij10, 673–681.

    Google Scholar 

  • Ratner, E. I., and S. F. Ukhina, 1963: Transformation of externally absorbed amino acids in roots of maize (russisch, engl. Zusammenfassung). — Fiziol. Rastenij10, 393–399.

    Google Scholar 

  • Scheuermann, W., 1964:35S-Inkorporation in das Wurzelspitzenmeristem vonVicia faba. — Z. Naturforsch.19b, 434–438.

    Google Scholar 

  • Sharma, A., and A. K. Sharma, 1960: Amino acid interference in chromosome metabolism. — Nucleus, Cambridge3, 215–224.

    Google Scholar 

  • Street, H. E., 1966: The physiology of root growth. — Ann. Rev. Plant Physiol.17, 315–344.

    Google Scholar 

  • Street, H. E., J. C. Hughes and J. S. Lewis, 1960: Studies on the growth of excised roots X. Individual amino acids and acid hydrolysed casein as nitrogen sources for the growth of excised tomato roots. — New Phytol., England59, 273–287.

    Google Scholar 

  • Valle, E., and A. I. Virtanen, 1960: On the uptake and assimilation of D- and L-amino acids by pea and barley plants. — Suom. Kemistil, Ser. B,33, 201–204.

    Google Scholar 

  • Virtanen, A. J., and H. Linkola, 1957: On the effect of amino acids and amines on the growth and the form of the pea plant. — Suom. Kemistil. Ser. B,30, 220.

    Google Scholar 

  • Wang, D., and R. H. Burris, 1965: Carbon metabolism of glycine and serine in relation to the synthesis of organic acids and a guanosine derivative. — Plant Physiol., Lancaster40, 419–424.

    Google Scholar 

  • Waris, H., 1959: Neomorphosis in seed plants induced by amino acids. I.Oenanthe aquatica. — Physiol. Plantarum, Kopenhagen12, 753–766.

    Google Scholar 

  • White, P. R., 1937: Amino acids in the nutrition of excised tomato roots. — Plant Physiol., Lancaster12, 793–802.

    Google Scholar 

  • Woetz, S. S., 1963: Growth-modifying and antimetabolite effects of amino acids onChrysanthemum. — Plant Physiol., Lancaster38, 93–99.

    Google Scholar 

  • Wright, D. E., 1962: Amino acid uptake by plant roots. — Arch. Biochem. Biophys., New York97, 174–180.

    Google Scholar 

  • Younis, A. E., 1960: Uptake and utilization of L-glutamic acid by radish root slices. — Physiol. Plantarum, Kopenhagen13, 104–111.

    Google Scholar 

Download references

Authors
  1. Conrad Polter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Polter, C. Einfluß von Aminosäuren auf das Wachstumin vitro kultivierter Wurzeln vonPisum sativum L.. Die Kulturpflanze 17, 163–178 (1969). https://doi.org/10.1007/BF02097942

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02097942

Search

Navigation