Abstract
The glucose-utilizing mutantHydrogenomonas strain H16G+ differs from the original strain H16 in having a higher specific activity of glucose-6-phosphate dehydrogenase. During incubation of the original strain or of the mutant H16G+ in a mineral salts/fructose-medium under an atmosphere of 80% H2 + 20% O2, neither growth nor formation of the enzymes of the Entner-Doudoroff system occur. Molecular hydrogen represses the formation of these enzymes even in the presence of carbon dioxide, peptone, or lactate. Under air, the formation of the enzymes of the Entner-Doudoroff pathway is not repressed by lactate nor by acetate, glutamate or pyruvate. In strain H16G+ fructose suppresses the adaptation to glucose; glucose does not repress the formation of a fructose permease. Fructose also suppresses adaptation to and utilization of glutamate and aspartate, but not of lactate. In cells grown either chemolithotrophically or on fructose acetyl-CoA kinase, malate synthase and isocitrate lyase are rapidly formed under air after addition of acetate; the formation of these enzymes is also completely suppressed by molecular hydrogen or fructose.
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Schlegel, H.G., Trüper, H.G. Repression of enzyme formation inHydrogenomonas strain H16G+ by molecular hydrogen and by fructose. Antonie van Leeuwenhoek 32, 277–292 (1966). https://doi.org/10.1007/BF02097470
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DOI: https://doi.org/10.1007/BF02097470