Skip to main content
Log in

Deoxyribonucleic acid base composition and taxonomy of violet-pigmented cocci

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

The content of guanine and cytosine in DNA of the violet-pigmented micrococci designated asStaphylococcus flavocyaneus andMicrococcus flavocyaneus varies within the range of 70.8 – 72.0%. These species have similar deoxyribonucleic acid base compositions and do not differ physiologically and morphologically: they both produce yellow and violet pigments, hydrolyse gelatin and casein, reduce nitrates and do not form lipase. Therefore we consider them in accordance with Kocur and Martinec (1962, 1963) identical. They do not, however, seem to be identical withMicrococcus luteus (Schroeter, 1872) Cohn 1872 because the content of guanine and cytosine in DNA of the neotype culture of this species was found to be 66.3%.Micrococcus luteus differs from the violet pigmented micrococci also physiologically. It does not produce violet pigment, does not hydrolyse gelatin and casein and does not produce urease. For the violet pigmented strainMicrococcus cyaneus the use of the original designation is recommended:Micrococcus cyaneus (Schroeter) Cohn 1872, as it differs from the other violet cocci not only physiologically — it does not produce yellow pigment, oxidises mannitol, dulcitol and sorbitol, produces lipase and does not hydrolyse casein — but also in its DNA base composition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Boháček, J. andBlažíček, G. 1965. On the course of thermal denaturation of DNA afterγ-irradiation. Biophysik2 233–238.

    PubMed  Google Scholar 

  • Catlin, B. W. andCunningham, L. S. 1961. Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. J. Gen. Microbiol.26 303–312.

    PubMed  Google Scholar 

  • De Ley, J. andVan Muylem, J. 1963. Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek29 344–358.

    PubMed  Google Scholar 

  • De Ley, J. andSchell, J. 1963. Deoxyribonucleic acid base composition of acetic acid bacteria. J. Gen. Microbiol.33 243–253.

    PubMed  Google Scholar 

  • Doty, P., Marmur, J. andSueoka, N. 1959. The heterogeneity in properties and functioning of deoxyribonucleic acids. Brookhaven Symp. in Biology12 1–16.

    Google Scholar 

  • Doty, P., Marmur, J., Eigner, J., Schildkraut, C. 1960. Strand separation and specific recombination in deoxyribonucleic acids: physical chemical studies. Proc. Natl. Acad. Sci.46 461–476.

    Google Scholar 

  • Fredericq, E., Oth, A. andFontaine, F. 1961. The ultraviolet spectrum of deoxyribonucleic acids and their constituents. J. Mol. Biol.3 11–17.

    PubMed  Google Scholar 

  • Ki Yong Lee, Wahl, R. etBarbu, E. 1956. Contenu en bases puriques et pyrimidiques des acides désoxyribonucléiques des bactéries. Ann. Inst. Pasteur91 212–224.

    Google Scholar 

  • Kocur, M. andMartinec, T. 1962. Taxonomic study of the genusMicrococcus. Folia Přírod. fakulty UJEP3 1–121.

    Google Scholar 

  • Kocur, M. andMartinec, T. 1963. The classification of some violet-pigmented micrococci. J. Gen. Microbiol.32 185–188.

    PubMed  Google Scholar 

  • Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol.3 208–218.

    Google Scholar 

  • Marmur, J. andDoty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol.5 109–118.

    PubMed  Google Scholar 

  • Marmur, J., Falkow, S. andMandel, M. 1963. New approaches to bacterial taxonomy. Ann. Rev. Microbiol.17 329–372.

    Google Scholar 

  • Marmur, J., Seaman, E. andLevine, J. 1963. Interspecific transformation inBacillus. J. Bacteriol.85 461–467.

    Google Scholar 

  • Rosypal, S., Kocur, M. andHoďák, K. 1963. A study of violet-pigmented micrococci. Yellow-pigmented mutants ofStaphylococcus flavocyaneus. J. Gen. Microbiol.32 189–194.

    PubMed  Google Scholar 

  • Rosypalová, A. andRosypal, S. 1966. Some physiological properties of violet-pigmented micrococci. Their oxidation pattern and ability to grow in minimal synthetic medium. Publ. Fac. Sci. Univ. J. E. Purkyně, Brno.In press.

  • Posypalová, A., Boháček, J. andRosypal, S. 1966. Deoxyribonucleic acid base composition of some micrococci and sarcinae. Antonie van Leeuwenhoek32: in press.

  • Snedecor, G. W. 1956. Statistical methods. The Iowa State College Press, Ames, Iowa.

    Google Scholar 

  • Sueoka, N. 1961. Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data. J. Mol. Biol.3 31–40.

    Google Scholar 

  • Vendrely, R. 1958. La notion d'espèce à travers quelques données biochimiques récentes et le cycle L. Ann. Inst. Pasteur94 142–166.

    Google Scholar 

  • Wyatt, G. R. 1951. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem. J.48 584–590.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rosypalová, A., Boháček, J. & Rosypal, S. Deoxyribonucleic acid base composition and taxonomy of violet-pigmented cocci. Antonie van Leeuwenhoek 32, 105–112 (1966). https://doi.org/10.1007/BF02097450

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02097450

Keywords

Navigation