European Journal of Clinical Microbiology

, Volume 6, Issue 1, pp 64–67 | Cite as

Lysis centrifugation and slide blood culture systems for diagnosis of bacteremia in immunocompromised patients

  • H. Richet
  • M. C. Escande
  • P. H. Lagrange


Compared with a slide blood culture system, a lysis centrifugation system detected significantly more positive blood cultures and more episodes of bacteremia in immunocompromised patients (even when on antibiotics), while giving results more rapidly and even at significantly lower CFUs. However, it had a high contamination rate and did not detectPseudomonas aeruginosaas often. Use of the two systems together is recommended for diagnosing bacteremia early in immunocompromised patients.


Internal Medicine Blood Culture Culture System Immunocompromised Patient Positive Blood Culture 
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  1. 1.
    Richet, H., Gerbal, R., Hautefort, B., Chataigne, R., Lagrange, P. H.: Detection of bacteremia in leukemic patients by early subculture with a slide blood culture system. Pathologie et Biologie 1985, 33: 213–216.PubMedGoogle Scholar
  2. 2.
    Bille, J., Stockman, L., Roberts, G. D., Horstmeister, C. D., Ilstrup, D. M.: Evaluation of a lysis centrifugation system for recovery of yeasts and filamentous fungi from blood. Journal of Clinical Microbiology 1983, 18: 469–471.PubMedGoogle Scholar
  3. 3.
    Fleming, W. H. III, Lewis, B. H., Dorn, G. L.: Effectiveness of lysis centrifugation blood culture technique in recovering fungi from blood. Laboratory Medicine 1984, 15: 104–108.Google Scholar
  4. 4.
    Kelly, M. T., Buck, G. E., Fojtasek, G.: Evaluation of a lysis centrifugation and biphasic bottle blood culture system during routine use. Journal of Clinical Microbiology 1983, 18: 554–557.PubMedGoogle Scholar
  5. 5.
    Kiehn, T. E., Wong, B., Edouards, F. F., Armstrong, D.: Comparative recovery of bacteria and yeasts from lysis centrifugation and a conventional blood culture system. Journal of Clinical Microbiology 1983, 18: 300–304.PubMedGoogle Scholar
  6. 6.
    McLaughlin, J. C., Hamilton, P., Scholes, J. V., Bartlett, R. L.: Clinical laboratory comparison of lysis centrifugation and bactec radiometric blood culture technique. Journal of Clinical Microbiology 1983, 18: 1027–1031.PubMedGoogle Scholar
  7. 7.
    Henry, N. K., Grewell, C. M., Van Grevenhof, P. E., Ilstrup, D. M., Washington, J. A. II: Comparison of lysis centrifugation with a biphasic blood culture medium for the recovery of aerobic and facultative anaerobic bacteria. Journal of Clinical Microbiology 1984, 20: 413–416.PubMedGoogle Scholar
  8. 8.
    Dorn, G. L., Land, G. A., Wilson, G. E.: Improved blood culture technique based on centrifugation; clinical evaluation. Journal of Clinical Microbiology 1979, 9: 391–396.PubMedGoogle Scholar
  9. 9.
    Wing, E. F., Norden, C. W., Shadduck, R. K., Winkelstein, A.: Use of quantitative bacteriologic techniques to diagnose catheter-related sepsis. Archives of Internal Medicine 1979, 139: 482–483.CrossRefPubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • H. Richet
    • 1
  • M. C. Escande
    • 1
  • P. H. Lagrange
    • 1
  1. 1.Laboratoire Central de MicrobiologieHotel Dieu de ParisParis Cedex 04France

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