Advertisement

Current Microbiology

, Volume 20, Issue 6, pp 369–372 | Cite as

Biosynthesis of major phospholipids inCandida albicans

  • Nandini Mago
  • G. K. Khuller
Article

Abstract

Biosynthesis of major phospholipids was examined by identifying enzymes and in vitro uptake of specific labeled precursors through various pathways inCandida albicans. The presence of PS synthetase, choline kinase, and ethanolamine kinase was demonstrated in this organism. Phosphatidylcholine was found to be synthesized mainly through cytidine diphosphate-choline (CDP)-choline and methylation pathways. The presence of a methylation pathway was further confirmed by blocking methyltransferases with 2-hydroxyethyl hydrazine. Phosphatidylethanolamine was synthesized by all three, CDP-ethanolamine, Phosphatidylserine (PS)-decarboxylase, and base exchange pathways, while PS was formed by PS-synthetase and base exchange mechanisms.

Keywords

Choline Hydrazine Phosphatidylcholine Ethanolamine Phosphatidylethanolamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Carson MA, Emala M, Hogsten P, Waechter CJ (1984) Coordinate regulation of phosphatidylserine decarboxylase activity and phospholipid N-methylation in yeast. J Biol Chem 259:6267–6273PubMedGoogle Scholar
  2. 2.
    Chavant L, Dargent R, Rami J, Motant C (1980) Characterization of subcellular fractions, mitochondria and microsomes in two filameutous fungi. Biol Cell 37:299–303Google Scholar
  3. 3.
    Hokin LE, Hokin MR (1958) Phosphoinositides and protein secretion in pancreas slices. J Biol Chem 233:805–810PubMedGoogle Scholar
  4. 4.
    Homann MJ, Poole MA, Gaynor DM, Ho C, Carman GM (1987) Effect of growth phase on phospholipid biosynthesis inSaccharomyces cerevisiae. J Bacteriol 169:533–539PubMedGoogle Scholar
  5. 5.
    Homann MJ, Bailis AM, Henry SA, Carman GM (1987) Coordinate regutation of phospholipid biosynthesis by serine inSaccharomyces cerevisiae. J Bacteriol 169:3276–3280PubMedGoogle Scholar
  6. 6.
    Kanfer JN (1980) The base exchange enzymes and phospholipase D of mammalian tissue. Can J Biochem 58:1370–1380PubMedGoogle Scholar
  7. 7.
    Kasinathan C, Khuller GK (1983) Biosynthesis of major phospholipids inMicrosporum gypseum. Biochim Biophys Acta 752:187–190Google Scholar
  8. 8.
    Kuchler K, Duam G, Paltauf F (1986) Subcellular and submitochondrial localizations of phospholipid synthesizing enzymes in yeast. J Bacteriol 165:901–911PubMedGoogle Scholar
  9. 9.
    Nikawa J, Yamashita S (1981) Characterization of phosphatidylserine synthase fromSaccharomyces cerevisiae and a mutant defective in the enzyme. Biochim Biophys Acta 665:420–426PubMedGoogle Scholar
  10. 10.
    Nikawa J, Yamashita S (1983) 2-Hydroxyethlhydrazine as a potent inhibitor of phospholipid methylation in yeast. Biochim Biophys Acta 751:201–209PubMedGoogle Scholar
  11. 11.
    Paeletti R, Kritchevsky D (1982) Biosynthesis and transport of phosphatidylserine in the cells. Adv Lipid Res 19:163–183PubMedGoogle Scholar
  12. 12.
    Pelech SL, Vance DE (1984) Regulation of phosphatidylcholine biosynthesis. Biochim Biophys Acta 779:217–251PubMedGoogle Scholar
  13. 13.
    Sherr SI, Byc C (1971) Choline and serine incorporation into phospholipids ofNeurospora crassa. Biochim Biophys Acta 239:243–247PubMedGoogle Scholar
  14. 14.
    Skipski VP, Peterson BF, Barclay M (1964) Quantitative analysis of phospholipids by thin layer chromatography. Biochem J 90:374–378PubMedGoogle Scholar
  15. 15.
    Steiner MR, Lester RL (1972)In vitro studies of phospholipid biosynthesis inSaccharomyces cerevisiae Biochim Biophys Acta 260:222–243PubMedGoogle Scholar
  16. 16.
    Trivedi A, Dudani AK, Prasad R (1983) Why choline supplementation did not enhance phosphatidyl choline level inCandida albicans. Biochem. Int 6:119–128PubMedGoogle Scholar
  17. 17.
    Weinhold PA, Rethy VB (1972) Ethanolamine phosphokinase activity and properties during liver development. Biochim Biophys Acta 276:143–154PubMedGoogle Scholar
  18. 18.
    Weinhold PA, Reithy VB (1974) The separation and purification of ethanolamine kinase and choline kinase from rat liver. Biochemistry 13:5135–5141PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Nandini Mago
    • 1
  • G. K. Khuller
    • 1
  1. 1.Department of BiochemistryPostgraduate Institute of Medical Education and ResearchChandigarhIndia

Personalised recommendations