Advertisement

Current Microbiology

, Volume 20, Issue 6, pp 365–368 | Cite as

Transcription and in vivo expression of aMicrocystis aeruginosa plasmid

  • Wolfgang Schwabe
  • Thomas Hübschmann
  • Martin Meixner
  • Andreas Weihe
  • Thomas Börner
Article

Abstract

A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 ofEscherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized byE. coli.

Keywords

Polypeptide Specific Manner ofEscherichia Coli Translation Signal Resident Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523PubMedGoogle Scholar
  2. 2.
    Börner T, Schwabe W, Hübschmann T, Weihe A, Henning M, Kohl J-G (1989) Analyse der Plasmide von toxischen Stämmen des CyanobacteriumsMicrocystis aeruginosa. Wiss Z Humboldt-Univ Berlin R Math/Natwiss 38 (1):45–48Google Scholar
  3. 3.
    Cangelosi GA, Joseph CM, Rosen JJ, Meeks JC (1986) Cloning and expression of aNostoc sp leucin biosynthetic gene inE. coli. Arch Microbiol 145:315–321Google Scholar
  4. 4.
    Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  5. 5.
    Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267PubMedGoogle Scholar
  6. 6.
    Felkner RH, Barnum SR (1988) Plasmid content and homology of 16 strains of filamentous, nonheterocystous cyanobacteria. Curr Microbiol 17:37–41Google Scholar
  7. 7.
    Franche C, Reynaud PA (1986) Characterization of several tropical strains ofAnabaena andNostoc: morphological and physiological properties and plasmid content. Ann Inst Pasteur/Microbiol 137 A:179–197Google Scholar
  8. 8.
    Frazer AC, Curtiss R III (1975) Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol 69:1–84PubMedGoogle Scholar
  9. 9.
    Friedberg D, Seijffers J (1986) Controlled gene expression utilizingLambda phage regulatory signals in a cyanobacterium host (Anacystis nidulans). Mol Gen Genet 203(3):505–510PubMedGoogle Scholar
  10. 10.
    Golden SS, Sherman LA (1983) A hybrid plasmid is a stable cloning vector for the cyanobacteriumAnacystis nidulans R2. J Bacteriol 155:966–972PubMedGoogle Scholar
  11. 11.
    Gruber MY, Glick BR, Thompson JE, Gendel SM (1987)In vitro expression of a cyanobacterial plasmid. Curr Microbiol 15(5):265–268Google Scholar
  12. 12.
    Herrero A, Elhai J, Hohn B, Wolk CP (1984) Infrequent cleavage of clonedAnabaena variabilis DNA by restriction endonuclease fromA. variabilis. J Bacteriol 160:781–784PubMedGoogle Scholar
  13. 13.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of the bacteriophage T4. Nature 227:680–685PubMedGoogle Scholar
  14. 14.
    Lau RH, Doolittle WF (1979) Covalently closed circular DNAs in closely related unicellular cyanobacteria. J Bacteriol 137:648–652PubMedGoogle Scholar
  15. 15.
    Lau RH, Straus, NA. (1985) Versatile shuttle cloning vectors for the unicellular cyanobacteriumAnacystis nidulans R2. FEMS Microbiol Lett 27:253–256Google Scholar
  16. 16.
    Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163(1):16–20PubMedGoogle Scholar
  17. 17.
    Lönneborg A, Kalla SR, Samuelsson G, Gustafsson P, Öquist G (1988) Light-regulated expression of the psb A transcript in the cyanobacteriumAnacystis nidulans. FEBS Lett 240(1,2):110–114Google Scholar
  18. 18.
    Maniatis T, Fritsch EF, Sambrock J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor LaboratoryGoogle Scholar
  19. 19.
    Pühler A, Timmis KN (1984) Advanced molecular genetics. Berlin: Springer-VerlagGoogle Scholar
  20. 20.
    Rebiere M-C, Castets AM, Houmard J, Tandeau De Marsac N (1986) Plasmid distribution among unicellular and filamentous cyanobacteria: occurrence of large and mega-plasmids. FEMS Microbiol Lett 37:269–275Google Scholar
  21. 21.
    Schwabe W, Weihe A, Börner T, Henning M, Kohl J-G (1988) Plasmids in toxic and nontoxic strains of the cyanobacteriumMicrocystis aeruginosa. Curr Microbiol 17:133–137Google Scholar
  22. 22.
    Shestakov SV, Reaston J (1987) Gene-transfer and host-vector systems of cyanobacteria. Oxford Surveys Plant Mol Cell Biol 4:137–166Google Scholar
  23. 23.
    Shestakov SV, Elanskaya I, Bibikova M (1985) Vectors for gene cloning inSynechocystis sp. 6803. Abstract, p 109. V. Int Symposium on Photosynthetic Procaryotes, grindelwald, SwitzerlandGoogle Scholar
  24. 24.
    Tandeau De Marsac N, Houmard J (1987) Advance in cyanobacterial molecular genetics. In: Van Baalen C, Fay P (eds) The cyanobacteria: a comprehensive review. Amsterdam: Elsevier, pp 251–302Google Scholar
  25. 25.
    Van den Hondel CAMJJ, Keegstra W, Borrias WE, van Arkel GA (1979) Homology of plasmids in strains of unicellular cyanobacteria. Plasmid 2:323–333PubMedGoogle Scholar
  26. 26.
    Van den Hondel CAMJJ, van Leen RW, van Arkel GA, Duyvesteyn MGC, de Waard A (1983) Sequence-specific nucleases from the cyanobacteriumFremyella diplosiphon, and a peculiar resistance of its chromosomal DNA towards cleavage by other restriction enzymes. FEMS Microbiol Lett 16:7–12Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Wolfgang Schwabe
    • 1
  • Thomas Hübschmann
    • 1
  • Martin Meixner
    • 1
  • Andreas Weihe
    • 1
  • Thomas Börner
    • 1
  1. 1.Bereich Genetik der Sektion BiologieHumboldt Universität zu BerlinBerlinGDR

Personalised recommendations