Current Microbiology

, Volume 20, Issue 6, pp 365–368 | Cite as

Transcription and in vivo expression of aMicrocystis aeruginosa plasmid

  • Wolfgang Schwabe
  • Thomas Hübschmann
  • Martin Meixner
  • Andreas Weihe
  • Thomas Börner


A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 ofEscherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized byE. coli.


Polypeptide Specific Manner ofEscherichia Coli Translation Signal Resident Plasmid 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Wolfgang Schwabe
    • 1
  • Thomas Hübschmann
    • 1
  • Martin Meixner
    • 1
  • Andreas Weihe
    • 1
  • Thomas Börner
    • 1
  1. 1.Bereich Genetik der Sektion BiologieHumboldt Universität zu BerlinBerlinGDR

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