Current Microbiology

, Volume 20, Issue 6, pp 353–357 | Cite as

Biological activities of lipid components ofLeptospira interrogans serovarcopenhageni avirulent strain Shibaura

  • Norihisa Goto
  • Atsuko Moribayashi
  • Jun-ichi Maeyama
  • Yoshiko Arimitsu


The gas chromatogram showed that the polar lipids (PL) extracted fromLeptospira interrogans serovarcopenhageni avirulent strain Shibaura consisted mainly of palmitic acid, palmitoleic acid, and oleic acid, and that the free fatty acids (FFA) from the strain contained oleic acid at a concentration as high as 67.5%. The intracutaneous injection of FFA induced much stronger edematous and vascular permeability reactions in guinea pigs than did that of PL. In addition, O 2 generation of polymorphonuclear leukocytes in vitro was enhanced with FFA to much greater extent than with PL.

Among the FFA, oleic acid caused the strongest biological activities such as edematous reaction, vascular permeability reaction, O 2 -generating activity, and hemolytic activity. It is, therefore, very likely that oleic acid may play an important role in toxicities of leptospiral lipids.


Lipid Permeability Toxicity Biological Activity Free Fatty Acid 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Norihisa Goto
    • 1
  • Atsuko Moribayashi
    • 2
  • Jun-ichi Maeyama
    • 3
  • Yoshiko Arimitsu
    • 3
  1. 1.Department of General Biologics ControlNational Institute of HealthTokyoJapan
  2. 2.Laboratory of TechnologyNational Institute of HealthTokyoJapan
  3. 3.Department of Applied ImmunologyNational Institute of HealthTokyoJapan

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