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Current Microbiology

, Volume 20, Issue 6, pp 353–357 | Cite as

Biological activities of lipid components ofLeptospira interrogans serovarcopenhageni avirulent strain Shibaura

  • Norihisa Goto
  • Atsuko Moribayashi
  • Jun-ichi Maeyama
  • Yoshiko Arimitsu
Article

Abstract

The gas chromatogram showed that the polar lipids (PL) extracted fromLeptospira interrogans serovarcopenhageni avirulent strain Shibaura consisted mainly of palmitic acid, palmitoleic acid, and oleic acid, and that the free fatty acids (FFA) from the strain contained oleic acid at a concentration as high as 67.5%. The intracutaneous injection of FFA induced much stronger edematous and vascular permeability reactions in guinea pigs than did that of PL. In addition, O 2 generation of polymorphonuclear leukocytes in vitro was enhanced with FFA to much greater extent than with PL.

Among the FFA, oleic acid caused the strongest biological activities such as edematous reaction, vascular permeability reaction, O 2 -generating activity, and hemolytic activity. It is, therefore, very likely that oleic acid may play an important role in toxicities of leptospiral lipids.

Keywords

Lipid Permeability Toxicity Biological Activity Free Fatty Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. 1.
    Arean MV, Sarasin G, Green JH (1964) The pathogenesis of leptospirosis: toxin production byLeptospira icterohaemorrhagiae. Am J Vet Res 25:836–843PubMedGoogle Scholar
  2. 2.
    Arimitsu Y, Goto N, Akama K (1986) Skin reactions to sonic disrupted sample fromL. copenhageni strain Shibaura in guinea pigs. Abstracts, 23rd Annual Meeting, Japanese Leptospirosis Research Conference 1986:43–44Google Scholar
  3. 3.
    Arimitsu Y, Moribayashi A, Goto N (1989) Skin reaction to lipids from avirulent strain Shibaura ofLeptospira interrogans serovarcopenhageni. Can J Microbiol, 35:1009–1014PubMedGoogle Scholar
  4. 4.
    Bligh E, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917PubMedGoogle Scholar
  5. 5.
    Kakinuma K (1974) Effects of fatty acids on the oxidative metabolism of leukocytes. Biochim Biophys Acta 348:76–85PubMedGoogle Scholar
  6. 6.
    Mori M, Arimitsu Y, Otani S, Akama K (1974) Intracutaneous infection withLeptospira icterohaemorrhagiae (Shibaura strain) of the guinea pig. Jpn J Med Sci Biol 27:297–308PubMedGoogle Scholar
  7. 7.
    Stalheim OHV (1967) A toxin factor inLeptospira pomona. Proc Soc Exp Biol Med 126:412–415PubMedGoogle Scholar
  8. 8.
    Stalheim OHV (1968) Biological effects of leptospiral lipids. J Bacteriol 95:465–468PubMedGoogle Scholar
  9. 9.
    Surgala MJ, Beesley ED, Brubaker RR (1967) Influence of unsaturation on fibrinolytic activity of salts of fatty acids. Proc Soc Exp Biol Med 126:256–258PubMedGoogle Scholar
  10. 10.
    Van Gelder BF, Slater EC (1962) The extinction coefficient of cytochromeC. Biochim Biophys Acta 58:593–595PubMedGoogle Scholar
  11. 11.
    Vinh T, Adler B, Faine S (1986) Glycolipoprotein cytotoxin fromLeptospira interrogans serovarcopenhageni. J Gen Microbiol 132:111–123PubMedGoogle Scholar
  12. 12.
    Washida N, Sagawa A, Tamoto K, Koyama J (1980) Comparative studies on superoxide anion production by polymorphonuclear leukocytes stimulated with various agents. Biochim Biophys Acta 631:371–379PubMedGoogle Scholar
  13. 13.
    Watanabe J (1959) The reactions of skin tissues to the fatty acids in guinea pigs. Trans Soc Path Jpn 48:1375–1392Google Scholar
  14. 14.
    Weltzien HU (1973) Slow-reacting hemolytic phosphatides: benzylated lysolecithins. Biochim Biophys Acta 311:6–14PubMedGoogle Scholar
  15. 15.
    Yam PA, Miller NG, White RJ (1970) A leptospiral factor producing a cytopathic effect on L cells. J Infect Disease 122:310–317Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Norihisa Goto
    • 1
  • Atsuko Moribayashi
    • 2
  • Jun-ichi Maeyama
    • 3
  • Yoshiko Arimitsu
    • 3
  1. 1.Department of General Biologics ControlNational Institute of HealthTokyoJapan
  2. 2.Laboratory of TechnologyNational Institute of HealthTokyoJapan
  3. 3.Department of Applied ImmunologyNational Institute of HealthTokyoJapan

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