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Eine Anordnung zur Messung kleiner Sauerstoffaustauschraten photosynthetisierender Algen bei geringen Lichtintensitäten

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Zusammenfassung

Es wird eine Meßanordnung zur amperometrischen Registrierung der relativen Nettosauerstoffaustauschrate mittels einer membrangeschützten Platinelektrode beschrieben, die besonders für die Verwendung kleiner Versuchsobjekte (einzelliger Algen und isolierter Chloroplasten) geeignet ist. Die hohe Empfindlichkeit ermöglicht die Analyse von Sauer-stoffaustauschraten unter Verwendung monochromatischen Lichtes relativ hoher spektraler Reinheit. Die gute Stabilität des Elektrodenpotentials läßt eine große Verstärkung des Meßwertes und eine direkte Registrierung des Anstiegs der relativen Sauerstoffaustauschrate zu.

Summary

An amperometric device is described for the registration of relative oxygen exchange rates of small particles such as algae or isolated chloroplasts. The high sensitivity of the electrode according to the Clark-type make possible the application of monochromatic light with small half band wides for examination of photosynthetic reactions. The employed Ag/AgO-reference electrode has a very stable potential and allows a high amplification necessary for direct registration of the oxygen exchange rate derivatives. Some examples are given for registrated exchange rates.

Краткое содержание

Онисывается измерительный прибор для амперометрической регистрации относительной н¶rt;тто-дозы кислородного обмена с помощью ¶rt;лектрода, зещищенного мембраной. Этот прибор применяется, главным образом, для весьма малых обьектов, как напр. одноклеточные водоросли или изолированные хлоропласты. Его высокая чувствительность делает возможным анализ доз кислородного обмена при использовании монохроматического света относительно высокой спектральной чистоты. Большая стабильность электродного потенциала допускает значительное усиление величины измерения и непосредственную регистрацию возростания дозы кислородного обмена.

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Authors
  1. Klaus Adler
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Adler, K. Eine Anordnung zur Messung kleiner Sauerstoffaustauschraten photosynthetisierender Algen bei geringen Lichtintensitäten. Die Kulturpflanze 15, 151–160 (1967). https://doi.org/10.1007/BF02095710

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  • DOI: https://doi.org/10.1007/BF02095710

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