Current Microbiology

, Volume 20, Issue 1, pp 23–26 | Cite as

Characterization of the rhythmic nitrogen-fixing activity ofSynechococcus sp. RF-1 at the transcription level

  • Tan-Chi Huang
  • Te-Jin Chow


Synechococcus RF-1 synthesizes mRNA for nitrogenase continuously when the culture is growing in continuous light. The synthesis of the nitrogenase mRNA is cyclic and occurs exclusively during the dark periods when the culture grows in a diurnal light/dark regimen.Nif gene expression continues to take place in a cyclic pattern after the culture is transferred from a diurnal light/dark regimen to continuous light. The cyclic synthesis of mRNA for nitrogenase can be induced in a continuous light culture by periodic exposure of the culture to 3(3,4-dichlorophenol)-1,1-dimethylurea. Although Ca++ is necessary for the expression of nitrogenase activity inSynechococcus RF-1in vivo, the results of Northern blot assays indicate that the role of Ca++ is not at the transcription level.


Gene Expression Northern Blot Periodic Exposure Transcription Level Synechococcus 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Tan-Chi Huang
    • 1
  • Te-Jin Chow
    • 1
  1. 1.Institute of BotanyAcademia SinicaTaipeiTaiwan, ROC

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