European Journal of Pediatrics

, Volume 150, Issue 6, pp 416–418 | Cite as

Detection of multidrug-resistant protein, P-glycoprotein in childhood leukaemia and lymphoma

  • Y. Mizuno
  • T. Hara
  • M. Nagata
  • A. Tawa
  • T. Tsuruo
  • K. Ueda


Flow cytometric detection of surface P-glycoprotein, a multidrug-resistant gene product, with a monoclonal antibody, MRK 16, was performed on cells obtained from 18 children with leukaemia and lymphoma. Of 18 patients examined, 1 with malignant lymphoma at relapse showed a significant increase in P-glycoproteinpositive cells and a strong resistance to chemotherapy. Overexpression of P-glycoprotein in a case with B-cell type malignant lymphoma was confirmed by immunoprecipitation and Northern hybridization analysis. The present study suggests that an increased expression of surface P-glycoprotein might be involved in multidrug resistance at least in a certain case of childhood leukaemia and lymphoma.

Key words

P-glycoprotein Multidrug resistance Leukaemia Malignant lymphoma 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Carulli G, Petrini M, Marini A, Ambrogi F (1988) P-glycoprotein in acute leukemia and in the blastic crisis of myeloid leukemia. N Engl J Med 319:797–798Google Scholar
  2. 2.
    Ito Y, Tanimoto M, Kumazawa T, Okumura M, Morishima Y, Ohno R, Saito H (1989) Increased P-glycoprotein expression and multidrug-resistant gene (mdr1) amplification are infrequently found in fresh acute leukemia cells. Sequential analysis of 15 cases at initial presentation and relapsed stage. Cancer 63:1534–1538PubMedGoogle Scholar
  3. 3.
    Ma DDF, Scurr RD, Davy RA, Mackertich SM, Harman DH, Dowden G, Isbister JP, Bell DR (1987) Detection of multidrug resistant phenotype in acute non-lymphoblastic leukemia. Lancet I:135–137Google Scholar
  4. 4.
    Mattern J, Efferth T, Bak M, Ho AD, Volm M (1989) Detection of P-glycoprotein in human leukemias using monoclonal antibodies. Blut 58:215–217PubMedGoogle Scholar
  5. 5.
    Mizuno Y, Hara T, Nakamura M, Ueda K, Minakami S, Take H (1988) Classification of chronic granuromatous disease on the basis of monoclonal antibody-defined surface cytochrome b deficiency. J Pediatr 113:458–462PubMedGoogle Scholar
  6. 6.
    Pastan I, Gottesman M (1987) Multi-drug resistance in human cancer. N Engl J Med 316:1388–1393PubMedGoogle Scholar
  7. 7.
    Rosenberg ML, Mickley LA, Cole DE, Balis FM, Tsuruo T, Poplack D, Fojo AT (1989) Expression of mdr-1/P-170 gene in patients with acute lymphoblastic leukemia. Blood 74:1388–1395PubMedGoogle Scholar
  8. 8.
    Shimoyama M, Otsu T, Isida Y, Kuroki Y, Shimada Y, Hinai T, Minato K, Hamada H, Tsuruo T (1988) Detection of multidrug resistant phenotype in leukemia and lymphoma by monoclonal antibodies. Jpn J Cancer Chemother 15:2863–2870Google Scholar
  9. 9.
    Tsuruo T, Sugimoto Y, Hamada H, Roninsaon I, Okumura M, Adachi K, Morishima Y, Ohno R (1987) Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells. Jpn J Cancer Res 78:1415–1419PubMedGoogle Scholar
  10. 10.
    Ubezio P, Limonta M, D'Incalci M, Damia G, Masera G, Giudici G, Wolverto J, Beck WT (1989) Failure to detect the P-glycoprotein multidrug resistant phenotype in case of resistant childhood acute lymphoblastic leukemia. Eur J Cancer Clin Oncol 25:1895–1899PubMedGoogle Scholar
  11. 11.
    Volm M, Bak M, Efferth T, Lathan B, Mattern J (1988) Immunochemical detection of a resistance-associated glycoprotein in tissue culture cells, ascites tumors and human tumor xenografts by Mab 265/F4. Anticancer Res 8:531–536PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Y. Mizuno
    • 1
  • T. Hara
    • 1
  • M. Nagata
    • 1
  • A. Tawa
    • 2
  • T. Tsuruo
    • 3
  • K. Ueda
    • 1
  1. 1.Department of Paediatrics, Faculty of MedicineKyusyu UniversityFukuoka City 812Japan
  2. 2.Department of PaediatricsOsaka University HospitalOsakaJapan
  3. 3.Institute of Applied MicrobiologyUniversity of TokyoTokyoJapan

Personalised recommendations