European Journal of Pediatrics

, Volume 150, Issue 6, pp 416–418 | Cite as

Detection of multidrug-resistant protein, P-glycoprotein in childhood leukaemia and lymphoma

  • Y. Mizuno
  • T. Hara
  • M. Nagata
  • A. Tawa
  • T. Tsuruo
  • K. Ueda
Hematology/Oncology

Abstract

Flow cytometric detection of surface P-glycoprotein, a multidrug-resistant gene product, with a monoclonal antibody, MRK 16, was performed on cells obtained from 18 children with leukaemia and lymphoma. Of 18 patients examined, 1 with malignant lymphoma at relapse showed a significant increase in P-glycoproteinpositive cells and a strong resistance to chemotherapy. Overexpression of P-glycoprotein in a case with B-cell type malignant lymphoma was confirmed by immunoprecipitation and Northern hybridization analysis. The present study suggests that an increased expression of surface P-glycoprotein might be involved in multidrug resistance at least in a certain case of childhood leukaemia and lymphoma.

Key words

P-glycoprotein Multidrug resistance Leukaemia Malignant lymphoma 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Y. Mizuno
    • 1
  • T. Hara
    • 1
  • M. Nagata
    • 1
  • A. Tawa
    • 2
  • T. Tsuruo
    • 3
  • K. Ueda
    • 1
  1. 1.Department of Paediatrics, Faculty of MedicineKyusyu UniversityFukuoka City 812Japan
  2. 2.Department of PaediatricsOsaka University HospitalOsakaJapan
  3. 3.Institute of Applied MicrobiologyUniversity of TokyoTokyoJapan

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