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Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease

Digestive Diseases and Sciences volume 41, pages 2078–2086 (1996)Cite this article

Abstract

Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation whose cellular components are capable of oxidative respiratory bursts that may result in tissue injury. Mucosal biopsies were analyzed for protein carbonyl content (POPs), DNA oxidation products [8-hydroxy-2′-deoxyguanosine (8-OHdG)], reactive oxygen intermediates (ROIs), trace metals (copper, zinc, and iron) and superoxide dismutase (Cu-Zn SOD). In Crohn's disease biopsies, there was an increase in ROIs, POPs, 8-OHdG, and iron, while decreased copper and Cu-Zn SOD activity were found in inflamed tissues compared to controls. For ulcerative colitis, there was an increase in ROIs, POPs, and iron in inflamed tissue compared to controls, while decreased zinc and copper were observed. An imbalance in the formation of reactive oxygen species and antioxidant micronutrients may be important in the pathogenesis and/or perpetuation of the tissue injury in IBD and may provide a rationale for therapeutic modulation with antioxidants.

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Authors and Affiliations

  1. From the Departments of Medicine, Surgery, Pathology, Pediatrics, and the Research Laboratories of the Center for Pediatric Ileitis and Colitis of North Shore University Hospital-New York, University School of Medicine, Manhasset, New York

    Lisa Lih-Brody MD, Saul R. Powell PhD, Kevin P. Collier MD, Gautam M. Reddy MD, Renee Cerchia MS, Ellen Kahn MD, Gary S. Weissman MD, Seymour Katz MD, Robert A. Floyd PhD, Matthew J. McKinley MD, Stanley E. Fisher MD & Gerard E. Mullin MD

  2. the Free Radical Biology and Aging Research Program of the Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma

    Lisa Lih-Brody MD, Saul R. Powell PhD, Kevin P. Collier MD, Gautam M. Reddy MD, Renee Cerchia MS, Ellen Kahn MD, Gary S. Weissman MD, Seymour Katz MD, Robert A. Floyd PhD, Matthew J. McKinley MD, Stanley E. Fisher MD & Gerard E. Mullin MD

Authors
  1. Lisa Lih-Brody MD
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  2. Saul R. Powell PhD
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  3. Kevin P. Collier MD
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  4. Gautam M. Reddy MD
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  5. Renee Cerchia MS
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  6. Ellen Kahn MD
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  7. Gary S. Weissman MD
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  9. Robert A. Floyd PhD
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  11. Stanley E. Fisher MD
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  12. Gerard E. Mullin MD
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Additional information

This work was supported in part by the Joe and Emily Lowe Foundation, Reach Out for Youth with Ileitis and Colitis, and a gift from Ruth and Leonard Litwin. Dr. Mullin is the recipient of a Career Development Award from the Crohn's and Colitis Foundation of America and an Investigator's Award from the American College of Gastroenterology.

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Lih-Brody, L., Powell, S.R., Collier, K.P. et al. Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease. Digest Dis Sci 41, 2078–2086 (1996). https://doi.org/10.1007/BF02093613

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Key words

  • oxygen free radicals
  • inflammation
  • tissue injury
  • inflammatory bowel disease
  • antioxidants