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Release of nonesterified fatty acids during cerulein-induced pancreatitis in rats

Digestive Diseases and Sciences volume 41, pages 1959–1965 (1996)Cite this article

Abstract

During acute pancreatitis, data obtainedin vitro suggest that pancreatic lipase, acting on circulating or tissular triglycerides, might generate nonesterified fatty acids (NEFA) that could promote pancreatic and fat tissue necrosis. This work determined whether NEFA were actually producedin vivo in pancreatic tissue and in blood during cerulein-induced pancreatitis in rats. Intraperitoneal injections of cerulein induced pancreatitis. To promote the possible NEFA release by pancreatic lipase, a venous infusion of human very low density lipoprotein (VLDL) was used to cause hypertriglyceridemia. NEFA were measured in portal and aortic blood and in tissue extracts prepared from pancreas homogenates. NEFA did not increase either in peripheral or in portal blood. In pancreatic tissue, NEFA levels did not differ from controls. The major hypertriglyceridemia produced by human VLDL intravenous infusion neither altered the course of the disease nor promoted plasma NEFA release. The role commonly attributed to NEFA in acute pancreatitis seems questionable.

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Affiliations

  1. From the INSERM U410, Neuroendocrinologie et Biologie Cellulaire Digestives, Faculté Xavier Bichat, 16 rue Henri Huchard, 75018, Paris, France

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD &  Claude Rozé MD, PhD

  2. Anatomie Pathologique, Hôpital Beaujon, 100 avenue du Général Leclerc, 92118, Clichy, France

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD &  Claude Rozé MD, PhD

  3. Biochimie A, Hôpital Xavier Bichat, 46 rue Henri Huchard, 75018, Paris, France

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD &  Claude Rozé MD, PhD

Authors
  1. François Paye MD
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  2. Jacques Chariot PhD
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  3. Georges Molas MD
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  4. Joëlle Benessiano PhD
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  5. Claude Rozé MD, PhD
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Additional information

Dr. Paye was the recipient of a scholarship from the Fondation pour la Recherche Médicale (Paris). Partial financial support of the work was provided by the Conseil Scientifique of Faculté X. Bichat and by Association Charles Debray.

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Paye, F., Chariot, J., Molas, G. et al. Release of nonesterified fatty acids during cerulein-induced pancreatitis in rats. Digest Dis Sci 41, 1959–1965 (1996). https://doi.org/10.1007/BF02093596

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  • DOI: https://doi.org/10.1007/BF02093596

Key words

  • fatty acids
  • cerulein
  • pancreatitis
  • rat
  • hypertriglyceridemia