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Current Microbiology

, Volume 20, Issue 2, pp 105–110 | Cite as

Plasmid mobilization from genetically engineered bacteria to members of the indigenous soil microflora in situ

  • Rolf B. Henschke
  • Friedrich R. J. Schmidt
Article

Abstract

For study of gene transfer and expression in a large variety of soil bacteria in situ, a gene-promoter cassette was constructed and inserted into an expression vector that allowed expression and maintenance in a wide host range. The hybrid replicon was transformed into a mobilizer strain that transferred its DNA to other Gram-negative bacteria at very high rates. This genetically engineered microorganism (GEM) was introduced into nonsterile soil microcosms. Plasmid transfer from the introduced GEM to members of the native soil flora was observed in situ.

Keywords

Expression Vector Gene Transfer Host Range Native Soil Soil Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Rolf B. Henschke
    • 1
  • Friedrich R. J. Schmidt
    • 1
  1. 1.Federal Agricultural Research CenterInstitute of Soil BiologyBraunschweigFRG

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