Current Microbiology

, Volume 22, Issue 4, pp 221–224 | Cite as

Metabolism inhibition as a result of interaction of antibody with a membrane protein ofMycoplasma bovoculi

  • Bawa Awumbila
  • Ricardo F. Rosenbusch
Article

Abstract

The metabolism inhibition reaction is a test commonly used for the speciation of mycoplasmas. Cattle infected withMycoplasma bovoculi produce serum antibodies that are active in metabolism inhibition. For characterization of this reaction, affinity matrixes were prepared from lysates ofM. bovoculi bound to a monoclonal antibody that recognizes a 94,000 kDa membrane protein (p94) of the mycoplasma. These matrixes containing purified p94 were used to specifically remove metabolism-inhibiting activity from serum samples of cattle infected withM. bovoculi. The results provide evidence that p94 is the major and perhaps only target for the action of complement-independent, metabolism-inhibiting antibodies. This antibody reactivity may inhibit a vital membrane function associated with the p94 protein.

Keywords

Monoclonal Antibody Membrane Protein Serum Sample Serum Antibody Antibody Reactivity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Bradbury JM, Oriel CA, Jordan FTW (1976) Simple method for immunofluorescent identification of mycoplasma colonies. J Clin Microbiol 3:449–452Google Scholar
  2. 2.
    Gale JL, Kenny GE (1970) Complement-dependent killing ofMycoplasma pneumoniae by antibody: kinetics of the reaction. J Immunol 104:1175–1183Google Scholar
  3. 3.
    Gourlay RN, Thomas LH (1969) The isolation of large colony and T-strain mycoplasmas from cases of bovine keratoconjunctivitis. Vet Rec 84:416–417Google Scholar
  4. 4.
    Hurrell JGR (1982) Monoclonal hybridoma antibodies: techniques and applications. Boca Raton: CRC Press, p 51Google Scholar
  5. 5.
    Kenny GE (1979) Antigenic determinants. In: Barile MF, Razin S (eds) The mycoplasmas, vol 1, Cell Biology. New York: Academic Press, pp 351–377Google Scholar
  6. 6.
    Knudtson WU, Reed DE, Daniels G (1986) Identification of Mycoplasmatales in pneumonic calf lungs. Vet Microbiol 11:79–91Google Scholar
  7. 7.
    Krause DC, Baseman JB (1983) Inhibition ofMycoplasma pneumoniae hemadsorption and adherence to respiratory epithelium by antibodies to a membrane protein. Infect Immun 39:1180–1186Google Scholar
  8. 8.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685Google Scholar
  9. 9.
    Langford EV, Dorward WJ (1969) A mycoplasma isolated from cattle with infectious bovine keratoconjunctivitis. Can J Comp Med 33:275–279Google Scholar
  10. 10.
    Lupton HW, Reed DE (1980) Evaluation of experimental subunit vaccines for infectious bovine rhinotracheitis. Am J Vet Res 41:388–390Google Scholar
  11. 11.
    Pejler G, Lindahl U, Larm O, Scholander E, Sandgren E, Lundblad A (1988) Monoclonal antibodies specific for oligosaccharides prepared by partial nitrous acid deamination of heparin. J Biol Chem 263:5197–5201Google Scholar
  12. 12.
    Rima BK, Baczko K, Imagawa DT, Ter Meulen V (1987) Humoral immune response in dogs with old dog encephalitis and chronic distemper meningoencephalitis. J Gen Virol 68:1723–1735Google Scholar
  13. 13.
    Rosenbusch RF (1983) Influence of mycoplasma preinfection on the expression ofMoraxella bovis pathogenicity. Am J Vet Res 44:1621–1624Google Scholar
  14. 14.
    Rosenbusch RF, Knudtson WY (1980) Bovine mycoplasmal conjunctivitis: experimental reproduction and characterization of the disease. Cornell Vet 70:307–320Google Scholar
  15. 15.
    Salih BA, Rosenbusch RF (1986) Antibody response in calves experimentally infected or naturally exposed toMycoplasma bovoculi. Vet Microbiol 11:93–102Google Scholar
  16. 16.
    Salih BA, Rosenbusch RF (1988) Attachment ofMycoplasma bovoculi to bovine conjunctival epithelium and lung fibroblasts. Am J Vet Res 49:1661–1664Google Scholar
  17. 17.
    Taylor-Robinson D (1983) Metabolism inhibition tests. In: Razin S, Tully JG (eds) Methods in mycoplasmology, vol 1. New York: Academic Press, pp 411–417Google Scholar
  18. 18.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354Google Scholar
  19. 19.
    Washburn LR, Ramsay JR, Roberts LK (1985) Characterization of the metabolism inhibition antigen ofMycoplasma arthritidis. Infect Immun 49:357–364Google Scholar

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Bawa Awumbila
    • 1
  • Ricardo F. Rosenbusch
    • 1
  1. 1.Veterinary Medical Research Institute, College of Veterinary MedicineIowa State UniversityAmesUSA

Personalised recommendations