Skip to main content
Log in

Collagen binding by lactobacilli

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

125I-labeled type I collagen (Cn-I) binding of 92 fresh isolates and 18 type culture collection strains of lactobacilli was tested. More than 75% of the strains bound Cn-I. The binding was inhibited by excess of unlabeled Cn-I, gelatin, and was sensitive to proteinase K. Other proteins such as fibronectin and albumin and various carbohydrates such asD-galactose,D-fucose, andD-mannose did not inhibit the binding. Therefore, we propose binding of Cn-I to lactobacilli involving specific surface protein(s).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature Cited

  1. Ascencio F, Aleljung P, Olusanya O, Wadström T (1990) Type I and IV collagen and fibrinogen binding to aeromonas species isolated from various infections. Zbl Bakt 273:186–194

    Google Scholar 

  2. Axelsson L, Chung TC, Dobrogosz WJ, Lindgren SE (1989) Discovery of a broad spectrum antimicrobial substance produced byLactobacillus reuteri. Microb Ecol Health Dis 2:131–136

    Google Scholar 

  3. Baloda SB, Faris A, Fröman G, Wadström T (1985) Fibronectin binding to Salmonella strains. FEMS Microbiol Lett 28:1–5

    Google Scholar 

  4. Borriello SP, Welch AR, Barclay FE, Davies HA (1988) Mucosal association byClostridium difficile in the hamster gastrointestinal tract. J Med Microbiol 25:191–196

    PubMed  Google Scholar 

  5. Dickson JS, Koohmaraie M (1989) Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces. Appl Environ Microbiol 55:832–836

    PubMed  Google Scholar 

  6. Fröman G, Switalski LM, Faris A, Wadström T, Höök M (1984) Binding ofEscherichia coli to fibronectin; a mechanism of tissue adherence. J Biol Chem 259:14899–14905

    PubMed  Google Scholar 

  7. Gullmar B, Hjerten S, Wadström T (1988) Cell surface charge of lactobacilli and enterococci isolated from pig small intestine as studied by free zone electrophoresis: a methodological study. Microbios 55:183–192

    PubMed  Google Scholar 

  8. Holderbaum D, Hall GS, Ehrhart LA (1986) Collagen binding toStaphylococcus aureus. Infect Immun 54:359–364

    PubMed  Google Scholar 

  9. Höök M, Switalski LM, Wadström T, Lindberg M (1989) Interactions of pathogenic microorganisms with fibronectin. In: Mosher DF (ed) Biology of extracellular matrix: fibronectin. New York: Academic Press, pp. 295–308

    Google Scholar 

  10. Jones GW, Rutter JM (1974) The association of K88 antigen with haemagglutinating activity in porcine strains ofEscherichia coli. J Gen Microbiol 84:135–144

    PubMed  Google Scholar 

  11. Jonsson P, Lindberg M, Haraldsson I, Wadström T (1985) Virulence ofStaphylococcus aureus in a mastitis model: studies of alpha hemolysin, coagulase and protein A as possible virulence determinants with protoplast fusion and gene cloning. Infect Immun 49:765–769

    PubMed  Google Scholar 

  12. Markwell MAK (1982) A new solid state reagent to iodinate proteins. Anal Biochem 125:427–432

    PubMed  Google Scholar 

  13. Naito Y, Gibbons RJ (1988) Attachment ofBacterioides gingivalis to collagenous substrata. J Dent Res 67:1075–1080

    PubMed  Google Scholar 

  14. Ohtomo T, Yoshida K (1988) Adhesion ofStaphylococcus aureus to fibrinogen, collagen and lectin in relation to cell surface structure. Zbl Bakt Hyg 268:325–340

    Google Scholar 

  15. Paulsson M, Wadström T (1990) Vitronectin and type-I collagen binding byStaphylococcus aureus and coagulase-negative staphylococci. FEMS Microbiol Immunol 65:55–62

    Google Scholar 

  16. Pedersen K, Tannock GW (1989) Colonization of the gastrointestinal tract by lactobacilli. Appl Environ Microbiol 55:279–283

    PubMed  Google Scholar 

  17. Silva M, Jacobus NV, Deneke C, Gorbach SL (1987) Antimicrobial substance from a humanLactobacillus strain. Antimicrob Agents Chemother 31:1231–1233

    PubMed  Google Scholar 

  18. Switalski LM, Speziale P, Höök M (1989) Isolation and characterization of a putative collagen receptor fromStaphylococcus aureus strain Cowan I. J Biol Chem 35:21080–21086

    Google Scholar 

  19. Thiel S, Reid KBM (1989) Structures and functions associated with the group of mammalian lectins containing collagen-like sequences. FEBS 250(1):77–78

    Google Scholar 

  20. Visai L, Speciale P, Bozzini S (1990) Binding of collagens to an enterotoxigenic strain ofEscherichia coli. Infect Immun 58:449–455

    PubMed  Google Scholar 

  21. Wadström T, Andersson K, Sydow M, Axelsson L, Lindgren S, Gullmar B (1987) Surface properties of lactobacilli isolated from the small intestine of pigs. J Appl Bacteriol 62:513–520

    PubMed  Google Scholar 

  22. Westerlund B, Kuusela P, Risteli J, Vartio T, Rauvala H, Virkola R, Korhonen T (1989) The O75X adhesin of uropathogenicEscherichia coli is a type IV collagen-binding protein. Mol Microbiol 3:329–337

    PubMed  Google Scholar 

  23. Wiersma EJ, Fröman G, Johansson S, Wadström T (1987) Carbohydrate specific binding of fibronectin toVibrio cholerae cells. FEMS Microbiol Lett 44:365–369

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aleljung, P., Paulsson, M., Emödy, L. et al. Collagen binding by lactobacilli. Current Microbiology 23, 33–38 (1991). https://doi.org/10.1007/BF02092306

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02092306

Keywords

Navigation