Summary
The aldehyde-bisulfite-toluidine blue (ABT) reaction is a selective topo-optical test of vicinal-OH and amino-OH groups. The localization of cardiac glycoside was investigated morphologically. After digoxin the sarcolemma membranes, capillaries and sinus node showed strong basophilia and negative birefringence. The topo-optical reaction are useful for the histopathological examination. The new method gives the possibility to the digoxin intoxication with a high specificity.
Zusammenfassung
Die Aldehyd-bisulfit-Toluidinblau (ABT) Reaktion wird in Geweben als selektive topo-optische Testmethode der vicinalen-OH-Gruppen und der Amino-OH-Gruppen angewandt. Die Lokalisation der herzwirksamen Glycoside wurde morphologisch untersucht. Nach experimenteller Digoxinintoxikation wurde das Reaktionsprodukt in den Sarcolemma-Membranen, den Sinus-Knoten und an den Kapillaren lokalisiert. Für die topo-optische Reaktion ist die durch die orientierte Farbstoffbindung herbeigeführte starke Doppelbrechung kennzeichnend. Die Unterschiede in der Doppelbrechung bieten eine Möglichkeit zur neuartigen Analyse in der Histopathologie bei Glycosidvergiftung.
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Sótonyi, P., Somogyi, E. Comparative topo-optical investigation of cardiac glycoside localization. Z Rechtsmed 90, 87–94 (1983). https://doi.org/10.1007/BF02092292
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DOI: https://doi.org/10.1007/BF02092292