Current Microbiology

, Volume 23, Issue 2, pp 71–74 | Cite as

Production of colonization factor antigen II of enterotoxigenicEscherichia coli is subject to catabolite repression

  • Dolores G. Evans
  • Doyle J. EvansJr.
  • Tuomo K. Karjalainen
  • Chao-Hung Lee
Article

Abstract

Experiments were performed to study the effect of glucose on the production of the fimbrial colonization factor CFA/II of enterotoxigenicEscherichia coli (ETEC). The production of the CFA/II antigen was examined by electron microscopy, quantitative ELISA, and hemagglutination. The results showed that addition of 1% glucose to the growth medium drastically decreased CFA/II production, whereas addition of glycerol or sodium acetate did not have any effect. Bacteria grown in the presence of 1% glucose were essentially devoid of CFA/II fimbriae when examined under the electron microscope. Addition of 1 mM cAMP reversed the repressive effect of glucose, suggesting that the glucose suppression on CFA/II synthesis is via the mechanism of catabolite repression.

Keywords

Glucose Sodium Acetate Microscopy Electron Microscope 

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Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Dolores G. Evans
    • 1
  • Doyle J. EvansJr.
    • 1
  • Tuomo K. Karjalainen
    • 2
  • Chao-Hung Lee
    • 2
  1. 1.Department of MedicineBaylor College of Medicine and Veterans Affairs Medical CenterHoustonUSA
  2. 2.Department of PathologyIndiana University School of MedicineIndianapolisUSA

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