Current Microbiology

, Volume 23, Issue 2, pp 71–74 | Cite as

Production of colonization factor antigen II of enterotoxigenicEscherichia coli is subject to catabolite repression

  • Dolores G. Evans
  • Doyle J. EvansJr.
  • Tuomo K. Karjalainen
  • Chao-Hung Lee


Experiments were performed to study the effect of glucose on the production of the fimbrial colonization factor CFA/II of enterotoxigenicEscherichia coli (ETEC). The production of the CFA/II antigen was examined by electron microscopy, quantitative ELISA, and hemagglutination. The results showed that addition of 1% glucose to the growth medium drastically decreased CFA/II production, whereas addition of glycerol or sodium acetate did not have any effect. Bacteria grown in the presence of 1% glucose were essentially devoid of CFA/II fimbriae when examined under the electron microscope. Addition of 1 mM cAMP reversed the repressive effect of glucose, suggesting that the glucose suppression on CFA/II synthesis is via the mechanism of catabolite repression.


Glucose Sodium Acetate Microscopy Electron Microscope 
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Literature Cited

  1. 1.
    Adhya S, Garges S (1982) How cyclic AMP and its receptor protein act inEscherichia coli. Cell 29:287–289Google Scholar
  2. 2.
    Craviato A, Scotland SM, Rowewe B (1982) Hemagglutination activity and colonization factor antigens I and II enterotoxigenic and non-enterotoxigenic strains ofEscherichia coli isolated from humans. Infect Immun 36:189–197Google Scholar
  3. 3.
    de Crombrugghe B, Busby S, Buc H (1984) Cyclic AMP receptor protein: role in transcription activation. Science 224:831–838Google Scholar
  4. 4.
    Epstein W, Rothman-Denes LB, Hesse J. (1975) Adenosine 3′:5′-cyclic monophoshate as mediator of catabolite repression inEscherichia coli. Proc Natl Acad Sci USA 72:2300–2304Google Scholar
  5. 5.
    Evans DG, Evans Jr DJ (1978) New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenicEscherichia coli of serogroups O6 and O8. Infect Immun 21:638–647Google Scholar
  6. 6.
    Evans DG, Silver RP, Evans Jr DJ, Chase DG, Gorbach SL (1975) Plasmid-controlled colonization factor associated with virulence inEscherichia coli enterotoxigenic for humans. Infect Immun 12:656–667Google Scholar
  7. 7.
    Evans DG, Evans Jr DJ, Tjoa W (1977) Hemagglutination of human group A erythrocytes by enterotoxigenicEscherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun 18:330–337Google Scholar
  8. 8.
    Evans DG, Evans Jr DJ, Clegg S, Pauley JA (1979) Purification and characterization of the CFA/I antigen of enterotoxigenicEscherichia coli. Infect Immun 25:738–748Google Scholar
  9. 9.
    Evans DG, Evans Jr DJ, Clegg S (1980) Detection of enterotoxigenicEscherichia coli colonization factor antigen I in stool specimens by an enzyme-linked immunosorbent assay. J Clin Microbiol 12:738–743Google Scholar
  10. 10.
    Gorbach SL, Khurana CM (1972) ToxigenicEscherichia coli: a cause of infantile diarrhea in Chicago. N Engl J Med 287:791–795Google Scholar
  11. 11.
    Gorbach SL, Kean BH, Evans DG, Evans Jr DJ, Bessudo D. (1975) Travelers' diarrhea and toxigenicEscherichia coli. N Engl J Med 292:933–936Google Scholar
  12. 12.
    Hamers AM, Pel HJ, Willshaw GA, Kusters JG, van der Zeijst BAM, Gaastra W (1989) The nucleotide sequence of the first two genes of the CFA/I fimbrial operon of human enterotoxigenicEscherichia coli. Microb Pathogen 6:297–309Google Scholar
  13. 13.
    Honda T, Arita M, Miwatani T (1984) Characterization of new hydrophobic pili of human enterotoxigenicEscherichia coli: a possible new colonization factor. Infect Immun 43:959–965Google Scholar
  14. 14.
    Karjalainen T.K., Evans DG, So M, Lee C-H (1989) Molecular cloning and nucleotide sequence of the colonization factor antigen I gene ofEscherichia coli. Infect Immun 57:1126–1130Google Scholar
  15. 15.
    Karjalainen T.K., Evans DG, Evans Jr DJ, Lee C-H (1990) Catabolite repression of the colonization factor antigen I (CFA/I) operon of enterotoxigenicEscherichia coli. Submitted for publication.Google Scholar
  16. 16.
    Mooi FR, de Graaf FK (1985) Molecular biology of fimbriae of enterotoxigenicEscherichia coli. Curr Top Microbiol Immunol 118:119–138Google Scholar
  17. 17.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor LaboratoryGoogle Scholar
  18. 18.
    Smyth CJ (1982) Two mannose-resistant haemagglutinins on enterotoxigenicEscherichia coli of serotype O6:K15:H16 or H- isolated from travellers' and infantile diarrhoea. J Gen Microbiol 128:2081–2096Google Scholar
  19. 19.
    Thomas LV, McConnell MM, Rowe B, Field AM (1985). The possession of three novel coli surface antigens by enterotoxigenicEscherichia coli strains positive for the putative colonization factor PCF8775. J Gen Microbiol 131:2319–2326Google Scholar
  20. 20.
    Watson JD, Hopkins NH, Roberts JW, Steitz JA, Weiner AM (1987) Molecular biology of the gene. Menlo Park, California: The Benjamin/Cummings Publishing Co., pp 478–485Google Scholar

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Dolores G. Evans
    • 1
  • Doyle J. EvansJr.
    • 1
  • Tuomo K. Karjalainen
    • 2
  • Chao-Hung Lee
    • 2
  1. 1.Department of MedicineBaylor College of Medicine and Veterans Affairs Medical CenterHoustonUSA
  2. 2.Department of PathologyIndiana University School of MedicineIndianapolisUSA

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