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Current Microbiology

, Volume 21, Issue 5, pp 329–337 | Cite as

Cloning and expression of anAeromonas hydrophila chitinase gene inescherichia coli

  • Paul E. Roffey
  • John M. Pemberton
Article

Abstract

The gene encoding an extracellular chitinase fromAeromonas hydrophila has been cloned and expressed inEscherichia coli. Plasmid pJP2512 contained the smallest DNA insert (3.9 kb) producing chitinase. The chitinase gene is transcribed from its own promoter, producing a protein of Mr 96,000. The chitinase open reading frame, an estimated 2.6 kb in length, has been subcloned to a 3.0 kb fragment; however, this fragment does not carry the functional chitinase promoter. InE. coli the chitinase enzyme is unable to transverse the outer membrane, being secreted across the cytoplasmic membrane and accumulating in the periplasmic space.

Keywords

Enzyme Outer Membrane Chitinase Cytoplasmic Membrane Chitinase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1990

Authors and Affiliations

  • Paul E. Roffey
    • 1
  • John M. Pemberton
    • 1
  1. 1.Department of MicrobiologyUniversity of QueenslandBrisbaneAustralia

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