Current Microbiology

, Volume 20, Issue 3, pp 171–175 | Cite as

Pseudomonas aeruginosa outer membrane protein F produced inEscherichia coli retains vaccine efficacy

  • Janice M. Matthews-Greer
  • Dawn E. Robertson
  • Linda B. Gilleland
  • Harry E. GillelandJr.
Article

Abstract

Pseudomonas aeruginosa outer membrane protein F was purified by extraction from polyacrylamide gels of cell envelope proteins of anEscherichia coli strain expressing the cloned gene for protein F. Antisera directed against protein F purified fromP. aeruginosa PAO1 reacted with thisE. coli strain by immunofluorescence assay and immunoblotting, whereas these antisera were nonreactive withE. coli strains lacking thePseudomonas protein F gene. The protein F purified from thisE. coli strain was used to immunize mice by intramuscular injection of 10 µg of protein F preparation on days 1 and 14, followed by burn and challenge of the mice on day 28. As compared with control mice immunized withE. coli K-12 lipopolysaccharide, immunization with theE. coli-derived protein F afforded significant protection against subsequent challenge with heterologous Fisher-Devlin immunotype 5 and 6 strains ofP. aeruginosa. Antisera from mice immunized with theE. coli-derived protein F reacted at bands corresponding to protein F and 2-mercaptoethanol-modified protein F upon immunoblotting against cell envelope proteins of the PAO1, immunotype 5, and immunotype 6 strains ofP. aeruginosa and theE. coli strain containing the cloned F gene, but failed to react at these sites in anE. coli strain lacking the F gene. These data demonstrate thatP. aeruginosa protein F produced inE. coli through genetic engineering techniques retains its vaccine efficacy in the complete absence of anyP. aeruginosa lipopolysaccharide.

Keywords

Polyacrylamide Pseudomonas Control Mouse Pseudomonas Aeruginosa Genetic Engineering 

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Janice M. Matthews-Greer
    • 1
  • Dawn E. Robertson
    • 1
  • Linda B. Gilleland
    • 2
  • Harry E. GillelandJr.
    • 2
  1. 1.Biology DepartmentCentenary College of LouisianaShreveport
  2. 2.Department of Microbiology and ImmunologyLouisiana State University Medical Center, School of Medicine in ShreveportShreveportUSA

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