Abstract
Escherichia coli has two forms of catalases, HPI and HPII. Both enzymes, but mainly HPII, are induced in cells reaching the stationary growth phase or under anaerobic conditions and are repressed in the presence of glucose. The induction at the stationary phase is dependent onfnr, a gene that regulates the expression of anaerobically induced proteins. The inhibition by glucose is not affected by cyclic AMP (cAMP) but is reduced in acrp mutant. The results show that HPII belongs to the group of genes controlled by the Fnr protein and is catabolically repressed in a manner that is independent of cAMP.
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Literature Cited
Bachmann BJ (1987) Linkage map ofEscherichia coli K-12, edition 7. In: Neidhart FC (ed)Escherichia coli andSalmonella typhimurium. Cellular and molecular biology. Washington DC: American Society for Microbiology, pp 807–876
Bachmann BJ (1987) Derivation and genotypes of some mutant derivatives ofEscherichia coli K-12. In: Neidhart FC (ed)Escherichia coli andSalmonella typhimurium. Cellular and molecular biology. Washington DC: American Society for Microbiology, pp 1190–1219
Botsford JL (1981) Cyclic nucleotides in prokaryotes. Microbiol Rev 45:620–642
Chippaux M, Bonnefoy-Orth V, Ratouchniak J, Pascal MC (1981) Operon fusions in the nitrate reductase operon and study of the control genenirR inEscherichia coli. Mol Gen Genet 182:477–479
Davis BD, Dulbecco R, Eisen HN, Ginsberg HS (1980) Microbiology, 3rd edn. Philadelphia: Harper and Row
Dessein A, Schwartz M, Ullman A (1978) Catabolite repression inEscherichia coli mutants lacking cAMP. Molec Gen Genet 162:83–87
Hasan N, Dur IF (1974) Induction ofβ-galactosidase inLactobacillus plantarum. J Bacteriol 120:66–73
Hassan HM, Fridovich I (1977) Regulation of superoxide dismutase synthesis inEscherichia coli: glucose effect. J Bacteriol 132:505–510
Hassan HM, Fridovich I (1978) Regulation of the synthesis of catalase and peroxidase inEscherichia coli. J Biol Chem 253:6445–6450
Hill S (1985) Redox regulation of entericnif expression is independent of thefnr gene product. FEMS Microbiol Lett 29:5–9
Ishida A, Uchida K, Kurashige T (1979) Effect of glucose on the H2O2-induced synthesis of peroxidase inEscherichia coli. Kumamoto J Sci Biol 14:27–32
Lin ECC, Kuritzkes DR (1987) Pathways for anaerobic electron transport. In: Neidhardt FC (ed)Escherichia coli andSalmonella typhimurium. Cellular and molecular biology. Washington DC: American Society for Microbiology, pp 201–221
Loewen PC (1984) Isolation of catalase-deficientEscherichia coli mutants and genetic mapping ofkatE, a locus that affects catalase activity. J Bacteriol 157:622–626
Loewen PC, Switala J, Triggs-Raine BL (1985) Catalases HPI and HPII inEscherichia coli are induced independently. Arch Biochem Biophys 243:144–149
Magasanik B, Neidhardt FC (1987) Regulation of carbon and nitrogen utilization. In: Neidhardt FC (ed)Escherichia coli andSalmonella typhimurium. Cellular and molecular biology. Washington DC: American Society for Microbiology, pp 1318–1325
Meir E, Yagil E (1984) Catalase-negative mutants ofEscherichia coli. Curr Microbiol 11:13–18
Meir E, Yagil E (1985) Further characterization of the two catalases inEscherichia coli. Curr Microbiol 12:315–320
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
Morgan RW, Christman MF, Jacobson MF, Storz G, Ames BN (1986) Hydrogen peroxide-inducible proteins inSalmonella typhimurium overlap with heat shock and other stress proteins. Proc Natl Acad Sci USA 83:8059–8063
Richter HE, Loewen PC (1981) Induction of catalase inExcherichia coli by ascorbic acid involves hydrogen peroxide. Biochem Biophys Res Commun 100:1039–1046
Richter HE, Loewen PC (1982) Catalase synthesis inEscherichia coli is not controlled by catabolite repression. Arch Biochem Biophys 215:72–77
Shaw DJ, Guest JR (1982) Amplification and product identification of thefnr gene ofEscherichia coli. J Gen Microbiol 128:2221–2228
Shaw DJ, Rice DW, Guest JR (1983) Homology between CAP and Fnr, a regulator of anaerobic respiration inEscherichia coli. J Mol Biol 166:241–247
Ullman A, Dachnin A (1983) Role of cyclic AMP in bacteria. Adv Cyclic Nucleotide Res 15:1–53
Unden G, Guest JR (1984) Cyclic AMP and anaerobic gene expression inE. coli. FEBS Lett 170:321–325
Wanner BL, Kodaira R, Neidhardt FC (1978) Regulation oflac operon expression: reappraisal of the theory of catabolite repression. J Bacteriol 136:947–954
Winkelman JW, Clark DP (1986) Anaerobically induced genes ofEscherichia coli. J Bacteriol 167:362–367
Yeung KH, Chaloner-Larsson G, Yamazaki H (1976) Evidence against the involvement of adenosine 3′,5′-cyclic monophosphate in glucose inhibition ofβ-galactosidase inBacillus megaterium. Can J Biochem 54:854–865
Yoshpe-Purer Y, Henis Y, Yashphe J (1977) Regulation of catalase level inEscherichia coli K12. Can J Microbiol 23:84–91
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Meir, E., Yagil, E. Regulation ofEscherichia coli catalases by anaerobiosis and catabolite repression. Current Microbiology 20, 139–143 (1990). https://doi.org/10.1007/BF02091988
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DOI: https://doi.org/10.1007/BF02091988