Current Microbiology

, Volume 23, Issue 3, pp 149–152 | Cite as

Construction of direct selection vectors for use inBacillus sphaericus

  • Anton F. Ehrhardt
  • William F. BurkeJr.
Article
  • 22 Downloads

Abstract

Genetic manipulation in the mosquito larvicidal organismBacillus sphaericus has been hampered by a lack of effective molecular biological techniques and tools. Currently available vectors forBacillus subtilis are inappropriate as they are selected with antibiotics to whichB. sphaericus is naturally resistant. Construction of chimeras between theStaphylococcus aureus plasmid pE194 and theB. subtilis direct selection vector pBD214 permitted selection of plasmid transformants with erythromycin. As with pBD214, direct selection of recombinants is accomplished by insertional inactivation of the plasmid-borne thymidylate synthetase gene and subsequent selection with trimethoprim.

Keywords

Erythromycin Trimethoprim Genetic Manipulation Direct Selection Biological Technique 

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Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Anton F. Ehrhardt
    • 1
  • William F. BurkeJr.
    • 1
  1. 1.Department of MicrobiologyArizona State UniversityTempeUSA

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