Skip to main content
SpringerLink
Log in

Radiopacity additives in silicone stent materials reduce in vitro bacterial adherence

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

In vitro adherence of the nosocomial pathogensPseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, andCandida albicans to select radiopaque silicone compounds was lower than that observed for the base silicone (p<0.03). Except forE. coli ATCC 11775 andCandida albicans, all microorganisms showed significantly lower adherence to a silicone compound impregnated with tantalum in comparison with a silicone compound impregnated with barium sulfate (p<0.05). Surface hydrophobicity of the silicone compounds did not show a direct correlation with the concentration of radiopacity additives or with degree of bacterial adherence. Scatchard analyses of data indicated that the number of adherence sites forP. aeruginosa on the base silicone, BaSO4-silicone, and Ta-silicone were 9.2×106 per mm2, 6.1×106 per mm2, and 3.7×106 per mm2 respectively. As determined by the Langmuir adsorption isotherm, the dissociation constants for adheredP. aeruginosa to the base silicone, BaSO4-silicone, and Ta-silicone were 2.50×103 mm4, 1.45×103 mm4, and 6.27×103 mm4 respectively.Pseudomonas aeruginosa demonstrated first order kinetics of adherence to the silicone compounds with a half saturation time of 4.15 h for the base silicone, 1.06 h for the BaSO4-silicone, and 2.14 h for the Ta-silicone. The use of Ta-silicone stents may delay the development of ascending urinary tract infections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Bisno AL, Waldvogel FA (1990) Infections associated with indwelling medical devices. Washington, DC: American Society for Microbiology

    Google Scholar 

  2. CDC (1981) Guidelines for prevention of catheter-associated urinary tract infections, 5. In: Guidelines for prevention and control of nosocomial infections. Atlanta, Georgia: Centers for Disease Control, pp 1–5

    Google Scholar 

  3. Cheung AL, Fischetti VA (1990) The role of fibrinogen in staphylococcal adherence to catheters in vitro. J Infect Dis 161:1177–1186

    PubMed  Google Scholar 

  4. Christensen GD, Simpson WA, Bisno AL, Beachey EH (1982) Adherence of slime producing strains ofStaphylococcus epidermidis to smooth surfaces. Infect Immun 37:318–326

    PubMed  Google Scholar 

  5. Costerton JW, Irvin RT (1981) The bacterial glycocalyx in nature and disease. Annu Review Microbiol 35:299–324

    Google Scholar 

  6. Daifuku R, Stamm WE (1986) Bacterial adherence to bladder uroepithelial cells in catheter-associated urinary tract infection. N Eng J Med 314:1208–1213

    Google Scholar 

  7. Doyle RJ, Oakley JD, Murphy KR, McAlister D, Taylor KG (1985) Graphical analyses of adherence data. In: Mergenhagen SE, Rosan B (eds) Molecular basis of oral microbial adherence. Washington, DC: American Society for Microbiology, pp. 109–113

    Google Scholar 

  8. Evans RC, Holmes CL (1987) Effect of vancomycin hydrochloride onStaphyloccus epidermidis biofilm associated with silicone elastomer. Antimicrob Agents Chemother 31:889–894

    PubMed  Google Scholar 

  9. Harris JM, Martin LF (1987) An in vitro study of the properties influencingStaphylococcus epidermidis adhesion to prosthetic vascular graft materials. Ann Surg 206:612–620

    PubMed  Google Scholar 

  10. Hessen MT, Kaye D (1989) Infections associated with foreign bodies in urinary tract. In: Bisno AL, Waldvogel FA (eds) Infections associated with indwelling medical devices. Washington, DC: American Society for Microbiology, pp. 119–213

    Google Scholar 

  11. Hogt AH, Dankert J, Hulstaert CE, Feijen J (1986) Cell surface characteristics of coagulase-negative staphlococciand their adherence to fluorinated poly(ethylenepropylene). Infect Immun 51:294–301

    PubMed  Google Scholar 

  12. Horan T, Culver D, Jarvis W, Emori G, Banerjee S, Martone W, Thornsberry C (1988) Pathogens causing nosocomial infections: preliminary data from the national nosocomial infections surveillance system. Antimicrob Newslett 5:65–67

    Google Scholar 

  13. Johnson GM, Lee DA, Regelmann WE, Gray ED, Peters G, Quie PG (1986) Interference with granulocyte function byStaphylococcus epidermidis slime. Infect Immun 54:13–20

    PubMed  Google Scholar 

  14. Jose MV, Larralde C (1982) Alternative interpretations of unusual Scatchard plots: contributions of interactions and heterogeneity. Math Biosci 58:159–170

    Google Scholar 

  15. Klotz IM (1982) Numbers of receptor sites from Scatchard graphs: facts and fantasies. Science 217:1247–1249

    PubMed  Google Scholar 

  16. Kunin CM, Fickelberg Z (1971) Evaluation of an intrauretheral lubricating catheter in prevention of catheter induced urinary tract infections. J Urol 106:928–930

    PubMed  Google Scholar 

  17. Litsky W (1990) Wanted: plastics with antimicrobial properties. Am J Pub Health, 80:13–15

    PubMed  Google Scholar 

  18. Mahrous M, Lott TJ, Meyer SA, Sawant AD, Ahearn DG (1990) Electrophoretic karyotypes of typical and atypicalCandida albicans. J Clin Microbiol 28:876–881

    PubMed  Google Scholar 

  19. McManus AT, Denton CL, Mason AD (1984) Topical chlorhexidine diphosphanilate (WP-973) in burn wound sepsis. Arch Surg 119:206–211

    PubMed  Google Scholar 

  20. Miller MJ, Ahearn DG (1987) Adherence ofPseudomonas aeruginosa to hydrophilic contact lenses and other substrata. J Clin Microbiol 25:1392–1397

    PubMed  Google Scholar 

  21. Miller MJ, Wilson LA, Ahearn DG (1988) Effects of protein, mucin, and human tears on adherence ofPseudomonas aeruginosa to hydrophilic contact lenses. J. Clin. Microbiol. 26:513–517

    PubMed  Google Scholar 

  22. Monson T, and Kunin CM (1974) Evaluation of a polymercoated indwelling catheter in prevention of infection. J Urol 111:220–222

    PubMed  Google Scholar 

  23. Nesbitt WE, Doyle RJ, Taylor KG, Statt RH, Arnold RR (1982) Positive cooperativity in the binding ofStreptococcus sanguis to hydroxylapatite. Infect Immun 35:157–165

    PubMed  Google Scholar 

  24. Nickel JC, Ruseska I., Wright JB, Costerton JB (1985) Tobramycin resistance ofPseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob Agents Chemother 27:619–624

    PubMed  Google Scholar 

  25. Peters G (1988) New considerations in the pathogenesis of coagulase-negative Staphylococcal foreign body infections. J Antimicrob Chemother 21:139–148

    PubMed  Google Scholar 

  26. Rosenberg M, Gutnick DL, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a useful technique for studying cell surface hydrophobicity. FEMS Microbiol Lett 22:289–295

    Google Scholar 

  27. Ruggieri MR, Hanno PM, Levin RM (1987) Reduction of bacterial adherence to catheter surface with heparin. J Urol 138:423–426

    PubMed  Google Scholar 

  28. Schaeffer AJ, Story KO, Johnson SM (1988) Effect of silver oxide/trichloroisocyanuric acid antimicrobial urinary drainage system on catheter-associated bacteriuria. J Urol 139:69–73

    PubMed  Google Scholar 

  29. Sugarman B (1982) Adherence of bacteria to urinary catheters. Urol Res 10:37–40

    PubMed  Google Scholar 

  30. Tidd MJ, Gow JG, Pennington JH, Shelton J, Scott MR (1976) Comparison of hydrophilic polymer-coated latex, uncoated latex and PVC indwelling balloon catheters in the prevention of urinary infection. Br J Urol 48:285–291

    PubMed  Google Scholar 

  31. United States Pharmacopeia, 22nd edition (1985) Biological reactivity tests, in vitro: agar diffusion test. Rockville, Maryland: U.S. Pharmacopeial Convention, Inc., p. 1496

  32. Vanhaecke E, Remon JP, Moors M, Raes F, De Rudder D, Van Peteghem A (1990) Kinetics ofPseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: Role of cell surface hydrophobicity. Appl Environ Microbiol 56:788–795

    PubMed  Google Scholar 

  33. van Loosdrecht MCM, Lyklema J, Norde W, Zehnder AJB (1990) Influence of interfaces on microbial activity. Microbiol. Rev. 54:75–87

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta

    A. D. Sawant, Manal Gabriel &  D. G. Ahearn

  2. Bard Urological Division, C.R. Bard, Inc., Covington, Georgia, USA

    M. S. Mayo

Authors
  1. A. D. Sawant
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manal Gabriel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Mayo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. G. Ahearn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sawant, A.D., Gabriel, M., Mayo, M.S. et al. Radiopacity additives in silicone stent materials reduce in vitro bacterial adherence. Current Microbiology 22, 285–292 (1991). https://doi.org/10.1007/BF02091956

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02091956

Keywords

Search

Navigation