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Current Microbiology

, Volume 20, Issue 5, pp 343–347 | Cite as

Adherence ofStaphylococcus epidermidis to pharyngeal epithelial cells mediated by lipoteichoic acid

  • Tulsi D. Chugh
  • George M. Bahr
  • Sahar A. Essa
  • Gary J. Burns
Article

Abstract

Prior treatment of pharyngeal epithelial cells (PEC) with lipoteichoic acid (LTA) derived fromStaphylococcus epidermidis produced a marked inhibition of adherence of the homologous strain and two heterologous strains. The inhibition was dose dependent and saturable with 100 µg/ml of LTA. However, pretreatment of PEC with deacylated LTA did not block the adherence of the three strains tested. A similar but less marked blocking effect on the adherence ofS. epidermidis to PEC was also observed with LTAs derived fromS. aureus andStreptococcus pyogenes. On treatment of bacteria with substances capable of binding to LTA, such as polyclonal mouse anti-LTA antibodies or with human albumin, a marked inhibition of bacterial adherence was observed. Immunofluorescence studies showed that anti-LTA antiserum bound readily to the surface of bacterial cells. These findings provide clear evidence that the lipid component of LTA located on the bacterial surface is centrally involved in the adherence ofS. epidermidis to human mucosal cells.

Keywords

Lipid Epithelial Cell Albumin Bacterial Cell Prior Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Tulsi D. Chugh
    • 2
  • George M. Bahr
    • 2
  • Sahar A. Essa
    • 2
  • Gary J. Burns
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineKuwait UniversityKuwait
  2. 2.Department of Microbiology, Faculty of MedicineKuwait UniversitySafatKuwait

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