Current Microbiology

, Volume 20, Issue 5, pp 335–338 | Cite as

Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells

  • Augustin Svoboda
  • Petr Ouředníček
Article

Abstract

Yeast protoplasts may regenerate the cell wall and revert to cells if immobilized in a 2%–5% Ca-alginate gel and cultured in an osmotically stabilized medium. The method of protoplast immobilization and subsequent isolation from the gel is described in detail. The reversion yield is dependent of the actual gel concentration, gel shape (beads vs. sheets) and of a medium molarity, and it may be up to 90%. The morphology of the cell wall regeneration and morphology of reversion to the cell forms correspond to protoplast development in gelatin or agar gels.

Keywords

Cell Wall Agar Immobilization Alginate Gelatin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Draget KI, Myhre S, Skjak-Braek G, Ostgaard K (1988) Regeneration, cultivation and differentiation of plant protoplasts immobilized in Ca-alginate beads. J Plant Physiol 132:552–556Google Scholar
  2. 2.
    Farkaš V (1988) Polysaccharide metabolism. In: Rose AH, Harrison JS (eds) The yeasts, vol 3, 2nd edn. London: Academic Press, pp 317–366Google Scholar
  3. 3.
    Johansen A, Flink JM (1986) Immobilization of yeast cells by internal gelation of alginate. Enzyme Micro Technol 8:145–148Google Scholar
  4. 4.
    Klein J, Wagner F (1983) Methods for the immobilization of microbial cells. In: Chibata I, Wingard LB (eds) Immobilization of microbial cells. Orlando: Academic Press, pp 11–51Google Scholar
  5. 5.
    Linsefors L, Brodelius P (1985) Immobilization of plant protoplasts: viability studies. Plant Cell Rep 4:23–27Google Scholar
  6. 6.
    Nečas O, Svoboda A (1985) Cell wall regeneration and protoplast reversion. In: Peberdy JF, Ferenczy L (eds) Fungal protoplasts. New York, Basel: Marcel Dekker Inc, pp 115–133Google Scholar
  7. 7.
    Smidsrod O (1972) Molecular bases for some physical properties of alginates in the gel state. Faraday Disc Soc 57:263–274Google Scholar
  8. 8.
    Svoboda A, Piedra D (1983) Reversion of yeast protoplasts in media containing polyethylene glycol. J Gen Microbiol 129:3371–3377Google Scholar
  9. 9.
    Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol Bioeng 26:53–58Google Scholar
  10. 10.
    Vidoli R, Yamazaki H, Nasim A, Veliky IA (1982) A novel procedure for the recovery of hybrid products from protoplast fusion. Biotechnol Lett 4:781–784Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Augustin Svoboda
    • 1
  • Petr Ouředníček
    • 1
  1. 1.Department of Biology, Medical FacultyJ.E. Purkyne UniversityBrnoCSSR

Personalised recommendations