Current Microbiology

, Volume 20, Issue 5, pp 335–338 | Cite as

Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells

  • Augustin Svoboda
  • Petr Ouředníček


Yeast protoplasts may regenerate the cell wall and revert to cells if immobilized in a 2%–5% Ca-alginate gel and cultured in an osmotically stabilized medium. The method of protoplast immobilization and subsequent isolation from the gel is described in detail. The reversion yield is dependent of the actual gel concentration, gel shape (beads vs. sheets) and of a medium molarity, and it may be up to 90%. The morphology of the cell wall regeneration and morphology of reversion to the cell forms correspond to protoplast development in gelatin or agar gels.


Cell Wall Agar Immobilization Alginate Gelatin 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Augustin Svoboda
    • 1
  • Petr Ouředníček
    • 1
  1. 1.Department of Biology, Medical FacultyJ.E. Purkyne UniversityBrnoCSSR

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