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Current Microbiology

, Volume 20, Issue 5, pp 299–303 | Cite as

Isolation and characterization ofEscherichia coli O157:H7 strains in China

  • Jian-Guo Xu
  • Tai-Su Quan
  • Dong-Lou Xiao
  • Tang-Ring Fan
  • Le-Mi Li
  • Chang-Ao Wang
  • Wei Li
  • Han-Ming Liu
Article

Abstract

Five strains ofEscherichia coli O157:H7 were isolated from 486 stool specimens collected in 1986, 1987, and 1988 from patients with diarrhea in Xuzhou City, Jiangsu Province, China; 21 of the specimens were from patients with bloody diarrhea. The biochemical reactions of all five strains were almost identical with those of the well-knownE. coli O157:H7 strain 933. All of the strains were found to carry a 60 Md plasmid and two small plasmids. The plasmid DNA Hind 111 restriction patterns were identical. The strains were lysed byE. coli typing phage E1, E2, and E3, but not by E4 or E5. Data suggested that it might belong to a single phage or plasmid group. All strains produced vero toxin and caused diarrhea and death in infant rabbits and mice.

Keywords

Diarrhea Coli Typing Jiangsu Province Biochemical Reaction Restriction Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Jian-Guo Xu
    • 2
  • Tai-Su Quan
    • 1
  • Dong-Lou Xiao
    • 2
  • Tang-Ring Fan
    • 1
  • Le-Mi Li
    • 1
  • Chang-Ao Wang
    • 2
  • Wei Li
    • 1
  • Han-Ming Liu
    • 2
  1. 1.Laboratory of BacteriologyXuzhou Sanitary and Anti-Epidemic StationXuzhou CityPROC
  2. 2.Dept. of Microbiology, The Institute of Epidemiology and MicrobiologyChinese Academy of Preventive MedicineChangping, BeijingPROC

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