Summary
Epithelial cells from the lungs of chicken embryos after 9 days incubation had been grown in culture and observed with the light- and electron microscope. We used cover-glass-, monolayer- and organoid-cultures in several culture-mediums. The epithelium grows in coherent plaques in cover-glass- and in monolayer-cultures. From the monolayer culture growing on fibrin or agar the epithelium is separated easily for subsequent experiments. With the electron-microscope we visualized the morphology of epithelial adhesions. The in vitro epitheliums are compared with in vivo alveolar lining cells morphologically and their stage of development in vitro is discussed.
The epithelial cells in the organoid-cultures are preserved well even after four days of culture.
Zusammenfassung
Epithelzellen aus den Lungen 9tägiger Hühnerembryonen wurden in Deckglas-, Monolayer- und Stückkulturen unter verschiedenen Bedingungen gezüchtet und licht- oder elektronenmikroskopisch untersucht. Mit den beiden ersten Kulturmethoden gelingt es, ein Epithelwachstum in zusammenhängenden Platten zu erreichen. Die Monolayer-Kulturen scheinen besonders brauchbar zu sein, da Epithel isoliert zu gewinnen ist und beim Wachstum auf organischen Filmen (Fibrin, Agar) auch eine Weiterverarbeitung in toto möglich ist. Elektronenmikroskopisch gelingt es, das Substrat der epithelialen Adhäsion genau darzustellen. Die in vitro-Epithelien werden morphologisch mit den Alveolardeckzellen in vivo verglichen und ihre Differenzierungsstufe diskutiert. — Die Epithelzellen in der Stückkultur sind noch nach 4 Tagen gut erhalten und damit als vital anzusehen.
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Merker, H.J., Zimmermann, B. Das elektronenmikroskopische Bild der Epithelzellen aus embryonalen Hühnerlungen in der Gewebekultur. Beitr. Klin. Tuberk. 139, 155–169 (1969). https://doi.org/10.1007/BF02090945
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DOI: https://doi.org/10.1007/BF02090945