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Inhibition of the calcitonin-induced outward current in identifiedAplysia neurons by interleukin-1 and interleukin-2

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Summary

1. The effects of bath-applied recombinant human interleukin-1 (rhIL-1) and interleukin-2 (rhIL-2) on the calcitonin (CT)-induced outward current recorded from identified neurons (R9–R12) ofAplysia kurodai were investigated with conventional voltage-clamp and pressure ejection techniques.

2. Micropressure ejection of CT onto the soma of the neuron induced a slow outward current [I o(CT); 4–6 nA in amplitude, 30–40 sec in duration] associated with a decrease in input membrane conductance.

3.I o(CT) was increased by hyperpolarization.

4. The extrapolated reversal potential was +10 mV. Additionally,I o(CT) was sensitive to changes in (Na+)o but not to changes in (K+)o, (Ca2+)o, and (Cl−)o.

5. Micropressure-ejected forskolin produced a slow outward current similar to that induced by CT.

6. Bath-applied rhIL-1 and rhIL-2 (10–40 U/ml) reduced the CT-induced current in identifiedAplysia neurons without affecting the resting membrane conductance or the holding current.

7. The inhibitory effects of both cytokines on the current were completely reversible. Heat-inactivated rhIL-1 and rhIL-2 were without effect.

8. These results suggest that the immunomodulators, IL-1 and IL-2, can modulate the CT-induced outward current associated with a decrease in Na+ conductance in the nervous system ofAplysia. Therefore, the study suggests that these cytokines may also serve as neuromodulators.

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Authors and Affiliations

  1. Department of Physiology, Shimane Medical University, 693, Izumo, Japan

    Masashi Sawada & Mitsuyuki Ichinose

  2. Old Westbury Neuroscience Institute, State University of New York at Old Westbury, 11568, Old Westbury, New York

    George B. Stefano

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  1. Masashi Sawada
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  2. Mitsuyuki Ichinose
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  3. George B. Stefano
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Sawada, M., Ichinose, M. & Stefano, G.B. Inhibition of the calcitonin-induced outward current in identifiedAplysia neurons by interleukin-1 and interleukin-2. Cell Mol Neurobiol 14, 175–184 (1994). https://doi.org/10.1007/BF02090783

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