Abstract
Hepatitis C virus (HCV) is an important cause of liver injury following liver transplantation in adults. We hypothesized that the prevalence of HCV infection in children following liver transplantation would be lower than the prevalence in adults after liver transplantation because HCV-related liver disease leading to liver transplantation in children is low and children require less blood products than adults during transplantation. We therefore performed a cross-sectional study to determine the prevalence of HCV infection in children who had undergone liver transplantation. Serum samples were obtained from 62 of 65 (95.4%) consecutive patients surviving for more than six months after transplantation. Using a second-generation enzyme-linked, immunosorbent assay (ELISA-2) and a second-generation recombinant immunoblot assay (RIBA-II), antibodies to HCV were detected in 5.1% (3 of 59) of the subjects. Using a single-step, polymerase chain reaction (PCR), HCV RNA was detected in 6.2% (4 of 62). All HCV-positive children had undergone liver transplantation before the initiation of routine screening for HCV in blood donors; overall 30 patients were transplanted prior to routine screening of blood products for HCV. The prevalence of HCV in infants and children after liver transplantation in our study is substantially less than the rates reported in adults. This difference may be due, in part, to the lower volume of blood product exposure and to the fact that children, as opposed to adults, rarely have chronic HCV infection as a cause of end-stage liver disease.
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Poterrucha JJ, Rakela J, Lumeng L, Lee CH, Taswell HF, Weisner RH: Diagnosis of chronic hepatitis C virus after liver transplantation by the detection of viral sequences with polymerase chain reaction. Hepatology 15:42–45, 1992
Wright TL, Donegan E, Hsu HH, Ferrell L, Lake JR, Kim M, Combs C, Fennessy S, Roberts JP, Ascher NL: Recurrent and acquired hepatitis C viral infection in liver transplant recipients. Gastroenterology 103:317–322, 1992
El-Ashmawy L, Hassanein T, Gavaler JS, Van Theil DH: Prevalence of hepatitis C virus antibody in a liver transplantation population. Dig Dis Sci 37:1110–1115, 1992
Rochlani M, Lewis JH, Ramsey GE, Bontempo FA, Shah G, Bowman RA, Van Theil DH, Starzl TE: Hepatitis C testing: Comparison of Ortho's EIA and RIBA II tests in 1,182 patients undergoing primary liver transplantation. Am J Clin Pathol 98:8–12, 1992
Franza A, Miguet JP, Bresson-Hadni S, Coaquette A, Lab M, Njoya O, Vanhems D, Becker ML, Rouget C, Mantion G: Prevalence of hepatitis C virus antibodies in orthotopic liver transplantation: A sequential study of 72 patients. Transplant Proc 23:1506, 1991
Read AE, Donegan E, Lake J, Ferrell L, Galbraith C, Kuramoto IK, Zeldis JB, Ascher NL, Roberts J, Wright TL: Hepatitis C in patients undergoing liver transplantation. Ann Intern Med 114:282–284, 1991
Fèray C, Samuel D, Theirs V, Gigou M, Dichon F, Bismuth A, Reynes M, Maisonneuve P, Bismuth H, Brechot C: Reinfection of liver graft by hepatitis C virus after liver transplantation. J Clin Invest 89:1361–1365, 1992
Pereira BJG, Milford EL, Kirkman RL, Quan S, Sayre KR, Johnson PJ, Wilber JC, Levey AS: Prevalence of hepatitis C virus RNA in organ donors positive for hepatitis C antibody and in their organs. N Engl J Med 327:910–915, 1992
Roth D, Fernandez J, Cirroco R: Seroprevalence of antibodies to hepatitis C virus (anti-HCV) among cadaver organ donors (CDS) and evidence for viral transmission (abstract). J Am Soc Nephrol 2:815, 1991
Wreghitt TG, Gray JJ, Allain JP: Frequent transmission of hepatitis C virus by organ donation. Hepatology 16:47A, 1992, (abstract)
Chomczyniski P, Sacchi N: Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159, 1987
Ahmad N, Schiff GM, Baroudy BM: Detection of viremia by a one step polymerase chain reaction method in hepatitis C virus infection. Virus Res 30:303–313, 1993
Gretch DR, Wilson JJ, Carithers RL, Rosa CD, Han JH, Corey L: Detection of hepatitis C virus RNA: comparison of one stage polymerase chain reaction (PCR) with nested set PCR. J Clin Microbiol 31:289–291, 1993
Dussaix E, DePaillette L, Laurent-Puig P, Martres P, Lykavieris P, Bernard O, Alverez P: Hepatitis C virus infection in pediatric liver transplantation. Transplantation 55:795–798, 1993
Al-Faleh FZ, Ayoola A, Al-Jeffry M, Al-Rashed R, Al-Mofarreh M, Arif M, Ramia S, Al-Karawi M, Al-Shabrawy M: Prevalence of antibody to hepatitis C virus among Saudi Arabian children: A community-based study. Hepatology 14:215–218, 1991
Lai ME, DeVirgilis S, Argiolu F, Farci P, Mazzoleni AP, Lisci V, Rapicetta M, Clemene MG, Nurchis P, Arnone M: Evaluation of antibodies to hepatitis C virus in a long-term prospective study of posttransfusion hepatitis among thalassemic children: Comparison between first- and second-generation assay. J Pediatr Gastroenterol Nutr 16:458–464, 1993
Fink FM, Hockerschulz S, Mor W, Puchhammer-Stockl E, Hofmann H, Zoubek A, Pawlowsky J, Hocker P, Gadner H: Association of hepatitis C virus infection with chronic liver disease in paediatric cancer patients. Eur J Pediatr 152:490–492, 1993
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Accepted for poster presentation at the “Update on Hepatic Transplantation,” 1993 Annual Meeting of the AASLD.
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Nowicki, M.J., Ahmad, N., Heubi, J.E. et al. The prevalence of hepatitis C virus (HCV) in infants and children after liver transplantation. Digest Dis Sci 39, 2250–2254 (1994). https://doi.org/10.1007/BF02090380
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DOI: https://doi.org/10.1007/BF02090380