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Intragastric nicotine protects against 40% ethanol-induced gastric injury despite pretreatment with NG-Nitro-l-Arginine methyl ester or adrenal medullectomy in rats

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Abstract

We tested the hypotheses that the protective effect of intragastric nicotine against ethanol-induced gastric mucosal injury is dependent on endogenous nitric oxide or peripheral sympathoadrenal mechanisms. Rats were pretreated with NG-nitro-l-arginine methyl ester (3 mg/kg subcutaneous, 1 h prior to study) to block endogenous nitric oxide synthesis or with adrenal medullectomy (three weeks prior to study) to ablate the effect of the adrenal medulla. At 1-h intervals, vehicle or nicotine (4 mg/kg) and 40% ethanol were then given intragastrically. The total lengths of the linear gastric corpus mucosal lesions were measured unbiasedly. The protective effect of intragastric nicotine was not modified by either pretreatment. We conclude that the mechanism mediating intragastric nicotine protection against 40% ethanol-induced gastric mucosal injury is independent of endogenous nitric oxide or the adrenal medulla.

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Authors and Affiliations

  1. From the Research and Medical Services, Sepulveda Veterans Administration Medical Center, Center for Ulcer Research and Education, UCLA School of Medicine, 91343, Los Angeles, California

    Fumihiro Iwata MD, Kazuo Endoh MD &  Felix W. Leung MD

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  1. Fumihiro Iwata MD
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  2. Kazuo Endoh MD
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  3. Felix W. Leung MD
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Iwata, F., Endoh, K. & Leung, F.W. Intragastric nicotine protects against 40% ethanol-induced gastric injury despite pretreatment with NG-Nitro-l-Arginine methyl ester or adrenal medullectomy in rats. Digest Dis Sci 39, 347–352 (1994). https://doi.org/10.1007/BF02090207

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  • DOI: https://doi.org/10.1007/BF02090207

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