Current Microbiology

, Volume 21, Issue 1, pp 75–79 | Cite as

The effect of sporulation temperature on sporal characteristics ofBacillus subtilis A

  • James A. Lindsay
  • Larry E. Barton
  • Annette S. Leinart
  • H. Stuart Pankratz
Article

Abstract

Spores ofBacillus subtilis A were produced at different temperatures (23°–49°C) and examined for a number of sporal characteristics. Spore heat resistance increased with sporulation temperature to 45°C, with spores grown at 49°C showing a dramatic reduction in resistance. Spore crops showed biphasic thermal death curves whether enumerated on germination medium with or without calcium dipicolinate. This strain produces both rough and smooth variants. Of the spores produced at 23°C, 99% were rough, had a density of 1.305, and an average core/core + cortex volume ratio of 0.1838. At 49°C, 99% were smooth, had a density of 1.275, and an average volume ratio of 0.3098. Between these temperatures both spore types were produced. There appeared to be no direct correlation with sporulation temperature, heat resistance, and dipicolinate content. There was an increase in both the magnesium and calcium contents to 45°C with a dramatic reduction at 49°C. The 1.305 density spores had higher calcium and dipicolinate contents than the 1.275 spores, although both spore types showed biphasic thermal death curves. The mechanisms involved in determining which spore type (rough/smooth) is produced at a specific growth temperature is unknown.

Keywords

Volume Ratio Growth Temperature Specific Growth Average Volume Heat Resistance 

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Literature Cited

  1. 1.
    Algie JE (1983) The heat resistance of bacterial spores and its relationship to the contraction of the forespore protoplast during sporulation. Curr Microbiol 9:173–176Google Scholar
  2. 2.
    Beaman TC, Gerhardt P (1986) Heat resistance of bacterial spores correlated with protoplast dehydration. mineralization and thermal adaptation. Appl Environ Microbiol 52:1242–1246PubMedGoogle Scholar
  3. 3.
    Beaman TC, Pankratz HS, Gerhardt P (1972) Ultrastructure of exosporium and underlying inclusions of spores ofBacillus megaterium. J. Bacteriol 109:1198–1209PubMedGoogle Scholar
  4. 4.
    Busta FF, Adams DM (1972) Identification of a germination system involved in the heat injury ofbacillus subtilis spores. Appl Microbiol 24:412–417PubMedGoogle Scholar
  5. 5.
    Busta FF, Ordal JZ (1964) Use of calcium dipicolinate for enumeration of total viable endospore populations without heat activation. Appl Microbiol 12:106–110PubMedGoogle Scholar
  6. 6.
    Edwards JL, Busta FF, Speck ML (1965) Thermal inactivation ofBacillus subtilis spores at high temperatures. Appl Microbiol 13:851–857PubMedGoogle Scholar
  7. 7.
    Edwards JL, Busta FF, Speck ML (1965) Heat injury ofBacillus subtilis spores at ultrahigh temperatures. Appl Microbiol 13:858–864PubMedGoogle Scholar
  8. 8.
    Gombas DE (1987) Bacterial sporulation and germination. In: Montville TJ (ed) Food microbiology, vol 1. Concepts in physiology and metabolism. Boca Raton, FL: CRC Press, pp 131–155Google Scholar
  9. 9.
    Khoury PH, Lombardi SJ, Slepecky RA (1987) Perturbation of the heat resistance of bacterial spores by sporulation temperature and ethanol. Curr Microbiol 15:15–19Google Scholar
  10. 10.
    Lechowich RV, Ordal ZJ (1962) The influence of the sporulation temperature on the heat resistance and chemical composition of bacterial spores. Can J Microbiol 8:287–295PubMedGoogle Scholar
  11. 11.
    Lindsay JA (1988) Characterization of bacterial spores from high-temperature growth transformants ofBacillus subtilis. Curr Microbiol 16:265–269Google Scholar
  12. 12.
    Lindsay JA, Murrell WG (1985) Changes in density of DNA after interaction with dipicolinic acid and its possible role in spore heat resistance. Curr Microbiol 12:329–334Google Scholar
  13. 13.
    Lindsay JA, Beaman TC, Gerhardt P (1985) Protoplast water content of bacterial spores determined by buoyant density sedimentation. J Bacteriol 163:735–737PubMedGoogle Scholar
  14. 14.
    Lindsay JA, Murrell WG, Warth AD (1985) Spore resístance and the basic mechanism of heat resistance. In: Harris LE, Skopek A (eds) Advances in sterilization of medical products, vol III. Sydney: Lindsay-Yates Press, pp 162–186Google Scholar
  15. 15.
    Murrell WG, Warth AD (1965) Composition and heat resistance of bacterial spores. In: Campbell LL, Halvorson HO (eds) Spores III. Ann Arbor, AI: American Society for Microbiology, pp 1–24Google Scholar
  16. 16.
    Stewart M, Somlyo AP, Somlyo AV, Shuman H, Lindsay JA, Murrell WG (1980) Distribution of calcium and other elements in cryosectionedbacillus cereus T spores, determined by high resolution scanning electron probe X-ray microanalysis. J Bacteriol 143:481–491PubMedGoogle Scholar
  17. 17.
    Warth AD (1983) Determination of dipicolinic acid in bacterial spores by derivative spectroscopy. Anal Biochem 130:502–505PubMedGoogle Scholar
  18. 18.
    Williams OB, Robertson WJ (1953) Effect of temperature of incubation at which formed on heat resistance of aerobic thermophilic spores. J Bacteriol 67:377–378Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • James A. Lindsay
    • 2
  • Larry E. Barton
    • 2
  • Annette S. Leinart
    • 2
  • H. Stuart Pankratz
    • 1
  1. 1.Department of Microbiology and Public HealthMichigan State UniversityEast LansingUSA
  2. 2.Food Science and Human Nutrition DepartmentUniversity of FloridaGainesvilleUSA

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